Report
Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies
| العنوان: | Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies |
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| المؤلفون: | Wang, Xueying, Liu, Qinghua, Zhou, Li, Song, Zongcheng, Li, Jun |
| بيانات النشر: | ELSEVIER SCIENCE INC |
| سنة النشر: | 2023 |
| المجموعة: | Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR / 中国科学院海洋研究所机构知识库 |
| مصطلحات موضوعية: | Turbot, Black rockfish, Sperm activation medium, Reproductive Biology, Veterinary Sciences, TURBOT SCOPHTHALMUS-MAXIMUS, ONCORHYNCHUS-MYKISS WALBAUM, RAINBOW-TROUT, BIOCHEMICAL-COMPOSITION, MOTILITY, ACTIVATION, GLYCOLYSIS, QUALITY |
| الوصف: | Ovarian fluid is essential for successful fertilization by maintaining the viability, motility, and velocity of sperm. The organic compounds and inorganic ions in ovarian fluid significantly influence spermatozoa's motility, velocity, and longevity. However, the effect of ovarian fluid on sperm performance is limited in teleost fish. In this study, the effect of ovarian fluid on sperm performance and its components in external fertilization species (Scophthalmus maximus, turbot) and internal fertilization species (Sebastes schlegelii, black rockfish) was investigated using computer-assisted sperm analysis, high-performance liquid chromatography, and metabolome analysis. The ovarian fluid had a distinct and species-specific effect on both species. In the black rockfish, the ovarian fluid from turbot significantly increased sperm motility (74.07% +/- 4.09%), as well as VCL (45 +/- 1.67 mm/s), VAP (40.17 +/- 1.6 mm/s), and VSL (36.67 +/- 1.86 mm/s), and longevity (352 +/- 11.31 min) (P < 0.05). In the turbot, only the longevity (71.33 +/- 5.69 min) and fertilization rate (65.27% +/- 11.59%) showed significantly improvement (P < 0.05). The ovarian fluid was rich in organic compounds, suggesting enrichment in the glycolysis/gluconeogenesis pathways. The results suggest that glycometabolism plays a crucial role in improving sperm performance in teleost with internal fertilization. Thus, incorporating ovarian fluid into the sperm activation medium can enhance artificial fertilization in fish breeding.(c) 2023 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | THERIOGENOLOGY; http://ir.qdio.ac.cn/handle/337002/182264; http://ir.qdio.ac.cn/handle/337002/182265 |
| DOI: | 10.1016/j.theriogenology.2023.05.003 |
| الاتاحة: | http://ir.qdio.ac.cn/handle/337002/182264 http://ir.qdio.ac.cn/handle/337002/182265 https://doi.org/10.1016/j.theriogenology.2023.05.003 |
| رقم الانضمام: | edsbas.60429E39 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.theriogenology.2023.05.003 |
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