Academic Journal
In vitro penetration of vancomicin through biofilms of coagulase negative staphylococci isolated from a hospital of Lima-Peru ; PENETRACIÓN IN VITRO DE VANCOMICINA EN BIOPELÍCULAS DE ESTAFILOCOCOS COAGULASA NEGATIVOS AISLADOS DE UN HOSPITAL DE LIMA-PERÚ
| العنوان: | In vitro penetration of vancomicin through biofilms of coagulase negative staphylococci isolated from a hospital of Lima-Peru ; PENETRACIÓN IN VITRO DE VANCOMICINA EN BIOPELÍCULAS DE ESTAFILOCOCOS COAGULASA NEGATIVOS AISLADOS DE UN HOSPITAL DE LIMA-PERÚ |
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| المؤلفون: | León, Sergio D., Rosas, Angela R., Salazar, María E. |
| المصدر: | Ciencia e Investigación; Vol. 19 Núm. 2 (2016); 84-88 ; Ciencia e Investigación; Vol. 19 No. 2 (2016); 84-88 ; 1609-9044 ; 1561-0861 |
| بيانات النشر: | Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica |
| سنة النشر: | 2017 |
| المجموعة: | Universidad Nacional Mayor de San Marcos: Revistas de investigación UNMSM |
| مصطلحات موضوعية: | Biofilms, coagulase negative staphylococci, vancomycin, Biopelículas, estafilococos coagulasa negativos, vancomicina |
| الوصف: | Infections associated with biofilm producer bacteria, such as coagulase negative staphylococci (CoNS) are difficult to treat, because they are more resistant to antibiotics than its plactonic form, perhaps because do not allow an optimal drug diffusion. This paper aims contribute to knowledge of resistance strategies by these microbial communities, considering as objectives, isolate biofilm producer CoNS from venous catheters(CVC), induce biofilms in vitro production and evaluate vancomycin penetration through of them. 121 CVC were collected, from Intensive Care Unit of Hospital Nacional Guillermo Almenara Irigoyen. 46 strains were identified as CoNS, being 46% of them, biofilm producers according to Congo Red agar method; Staphylococcus epidermidis ATCC 35984 (biofilm forming bacteria) and Staphylococcus epidermidis ATCC 12228 (non-biofilm forming bacteria) were used as controls. Vancomycin penetration through biofilms induced in vitro was evaluated by the Ander et al. method, observing that the antibiotic penetrated about 57,5% of its concentration through biofilms after six hours of exposure, while negative control diffused 92,4%. Almost, was observed that antibiotic continued to spread until 24 hours, reaching an average penetration of 82,2%. Statistical analysis showed no significant differences between counts of colonies of viable bacteria in the biofilms exposed to antibiotic and their respective controls. ; Las infecciones asociadas a bacterias formadoras de biopelículas, como estafilococos coagulasa negativos (ECoN), son difíciles de tratar debido a que en dicho estado son más resistentes a los antibióticos que en su forma planctónica, posiblemente porque no permiten la buena difusión del fármaco. El presente trabajo contribuye al conocimiento de las estrategias de resistencia por parte de estas comunidades microbianas, al plantearse como objetivos, aislar ECoN formadores de biopelículas desde catéteres venosos centrales (CVC), inducir la formación de biopelículas in vitro y evaluar la penetración de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Spanish; Castilian |
| Relation: | https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/13634/12038; https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/13634 |
| DOI: | 10.15381/ci.v19i2.13634 |
| الاتاحة: | https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/13634 https://doi.org/10.15381/ci.v19i2.13634 |
| Rights: | Derechos de autor 2017 Sergio D. León, Angela R. Rosas, María E. Salazar ; https://creativecommons.org/licenses/by-nc-sa/4.0 |
| رقم الانضمام: | edsbas.601DFF9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.15381/ci.v19i2.13634 |
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