التفاصيل البيبلوغرافية
| العنوان: |
Data from: Incubator-independent cell-culture perfusion platform for continuous long-term microelectrode array electrophysiology and time-lapse imaging |
| المؤلفون: |
Saalfrank, Dirk, Konduri, Anil Krishna, Latifi, Shahrzad, Habibey, Rouhollah, Golabchi, Asiyeh, Martiniuc, Aurel Vasile, Knoll, Alois, Ingebrandt, Sven, Blau, Axel |
| المصدر: |
10.5061/dryad.n55r8 ; oai:easy.dans.knaw.nl:easy-dataset:89224 ; oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:89224 ; 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c ; re3data_____::r3d100000044 ; 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 ; 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 ; 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f ; 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 ; 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 |
| بيانات النشر: |
Dryad Digital Repository |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
cell culture, benchtop perfusion, MEA electrophysiology, time lapse imaging (TLI), polydimethylsiloxane (PDMS), replica casting, Life sciences, medicine and health care, envir, socio |
| الوصف: |
Most in vitro electrophysiology studies extract information and draw conclusions from representative, temporally limited snapshot experiments. This approach bears the risk of missing decisive moments that may make a difference in our understanding of physiological events. This feasibility study presents a simple benchtop cell-culture perfusion system adapted to commercial microelectrode arrays (MEAs), multichannel electrophysiology equipment and common inverted microscopy stages for simultaneous and uninterrupted extracellular electrophysiology and time-lapse imaging at ambient CO2 levels. The concept relies on a transparent, replica-casted polydimethylsiloxane perfusion cap, gravity- or syringe-pump-driven perfusion and preconditioning of pH-buffered serum-free cell-culture medium to ambient CO2 levels at physiological temperatures. The low-cost microfluidic in vitro enabling platform, which allows us to image cultures immediately after cell plating, is easy to reproduce and is adaptable to the geometries of different cell-culture containers. It permits the continuous and simultaneous multimodal long-term acquisition or manipulation of optical and electrophysiological parameter sets, thereby considerably widening the range of experimental possibilities. Two exemplary proof-of-concept long-term MEA studies on hippocampal networks illustrate system performance. Continuous extracellular recordings over a period of up to 70 days revealed details on both sudden and gradual neural activity changes in maturing cell ensembles with large intra-day fluctuations. Correlated time-lapse imaging unveiled rather static macroscopic network architectures with previously unreported local morphological oscillations on the timescale of minutes. RSOS-150031 data filesData files accompanying manuscript 'Incubator-independent cell culture perfusion platform for continuous long-term microelectrode array electrophysiology and time-lapse imaging' by irk Saalfrank, Anil Krishna Konduri, Shahrzad Latifi, Rouhollah Habibey, Asiyeh ... |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://dx.doi.org/10.5061/dryad.n55r8; http://dx.doi.org/10.5061/dryad.n55r8 |
| DOI: |
10.5061/dryad.n55r8 |
| الاتاحة: |
https://doi.org/10.5061/dryad.n55r8 |
| Rights: |
lic_creative-commons |
| رقم الانضمام: |
edsbas.600DCC6B |
| قاعدة البيانات: |
BASE |