Academic Journal
Development and validation of HPLC and CE methods for simultaneous determination of amlodipine and atorvastatin in the presence of their acidic degradation products in tablets
| العنوان: | Development and validation of HPLC and CE methods for simultaneous determination of amlodipine and atorvastatin in the presence of their acidic degradation products in tablets |
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| المؤلفون: | Hassan Said A., Elzanfaly Eman S., El-Zeany Salem Badr A., Salem Maissa Y. |
| المصدر: | Acta Pharmaceutica, Vol 66, Iss 4, Pp 479-490 (2016) |
| بيانات النشر: | Sciendo |
| سنة النشر: | 2016 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | atorvastatin, amlodipine, capillary zone electrophoresis, hplc, stability, acidic degradation, Pharmaceutical industry, HD9665-9675 |
| الوصف: | Two methods were developed for separation and quantitation of amlodipine (AML) and atorvastatin (ATV) in the presence of their acidic degradation products. The first method was a simple isocratic RP-HPLC method while the second was capillary electrophoresis (CE). Degradation products were obtained by acidic hydrolysis of the two drugs and their structures were elucidated for the first time by IR and MS spectra. Degradation products did not interfere with the determination of either drug and the assays were therefore stability-indicating. The linearity of the proposed methods was established over the ranges 1-50 μg mL-1 for AML and ATV in the HPLC method and in the range of 3-50 and 4-50 μg mL-1 for AML and ATV, respectively, in the CE method. The proposed methods were validated according to ICH guidelines. The methods were successfully applied to estimation of AML and ATV in combined tablets. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1846-9558 |
| Relation: | https://doi.org/10.1515/acph-2016-0040; https://doaj.org/toc/1846-9558; https://doaj.org/article/f8f5771aa4d5482585e6722707aa56d2 |
| DOI: | 10.1515/acph-2016-0040 |
| الاتاحة: | https://doi.org/10.1515/acph-2016-0040 https://doaj.org/article/f8f5771aa4d5482585e6722707aa56d2 |
| رقم الانضمام: | edsbas.5FDBF95 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 18469558 |
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| DOI: | 10.1515/acph-2016-0040 |