Conference
Harnessing quorum sensing systems for complex genetic circuitry
| العنوان: | Harnessing quorum sensing systems for complex genetic circuitry |
|---|---|
| المؤلفون: | De Baets, Jasmine, Parmentier, Iréne, De Paepe, Brecht, De Mey, Marjan |
| المصدر: | 2024 Synthetic Biology: Engineering, Evolution & Design (SEED), Abstracts |
| سنة النشر: | 2024 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences |
| الوصف: | Quorum sensing serves as a communication technique utilized by bacteria to monitor the cell density in their environment. These quorum sensing systems have attracted a lot of attention as building blocks in the construction of genetic circuits, allowing the design of population-level regulated circuits, such as noise regulation or the creation of consortia comprising communicating bacterial strains. Moreover, these systems offer great possibilities to optimize pathway regulation by allowing the auto-inducible expression of a gene or pathway of interest, thereby circumventing the necessity of expensive inducers, such as IPTG, to induce a pathway at higher cell densities. In this research, two different quorum sensing systems, the LasR/LasI and EsaR/EsaI system, were researched. The systems were investigated and engineered for limited crosstalk facilitating their concurrent application within the same bacterial strain. Additionally, a novel promoter was created that is only activated in the presence of the auto-inducer molecules of both quorum sensing systems. This offers numerous new possibilities for genetic circuits outputs, with promising applications for pathway regulation. For example, a multistage switch, consisting of a growth stage followed by two production stages separated in time, holds great promise for the regulation of complex pathways competing with the central metabolism or exerting toxic effects. |
| نوع الوثيقة: | conference object |
| وصف الملف: | application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| اللغة: | English |
| Relation: | https://biblio.ugent.be/publication/01JA63JB5Z5YD9HTX505PNRTWM; https://biblio.ugent.be/publication/01JA63JB5Z5YD9HTX505PNRTWM/file/01JA653N3Q38J05Z5W2X39068Y |
| الاتاحة: | https://biblio.ugent.be/publication/01JA63JB5Z5YD9HTX505PNRTWM http://hdl.handle.net/1854/LU-01JA63JB5Z5YD9HTX505PNRTWM https://biblio.ugent.be/publication/01JA63JB5Z5YD9HTX505PNRTWM/file/01JA653N3Q38J05Z5W2X39068Y |
| Rights: | No license (in copyright) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.5FBBEE1F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |