Academic Journal
The non-muscle actinopathy-associated mutation E334Q in cytoskeletal γ-actin perturbs interaction of actin filaments with myosin and ADF/cofilin family proteins
| العنوان: | The non-muscle actinopathy-associated mutation E334Q in cytoskeletal γ-actin perturbs interaction of actin filaments with myosin and ADF/cofilin family proteins |
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| المؤلفون: | Greve, Johannes N, Marquardt, Anja, Heiringhoff, Robin, Reindl, Theresia, Thiel, Claudia, Di Donato, Nataliya, Taft, Manuel H, Manstein, Dietmar J |
| المساهمون: | Deutsche Forschungsgemeinschaft, Bundesministerium für Bildung und Forschung, Horizon 2020, Hannover Medical School, Norddeutscher Verbund für Hoch- und Höchstleistungsrechnen |
| المصدر: | eLife ; volume 12 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2024 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | Various heterozygous cytoskeletal γ-actin mutations have been shown to cause Baraitser–Winter cerebrofrontofacial syndrome, non-syndromic hearing loss, or isolated eye coloboma. Here, we report the biochemical characterization of human cytoskeletal γ-actin carrying mutation E334Q, a mutation that leads to a hitherto unspecified non-muscle actinopathy. Following expression, purification, and removal of linker and thymosin β4 tag sequences, the p.E334Q monomers show normal integration into linear and branched actin filaments. The mutation does not affect thermal stability, actin filament nucleation, elongation, and turnover. Model building and normal mode analysis predict significant differences in the interaction of p.E334Q filaments with myosin motors and members of the ADF/cofilin family of actin-binding proteins. Assays probing the interactions of p.E334Q filaments with human class 2 and class 5 myosin motor constructs show significant reductions in sliding velocity and actin affinity. E334Q differentially affects cofilin-mediated actin dynamics by increasing the rate of cofilin-mediated de novo nucleation of actin filaments and decreasing the efficiency of cofilin-mediated filament severing. Thus, it is likely that p.E334Q-mediated changes in myosin motor activity, as well as filament turnover, contribute to the observed disease phenotype. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.93013 |
| الاتاحة: | http://dx.doi.org/10.7554/elife.93013 https://cdn.elifesciences.org/articles/93013/elife-93013-v1.pdf https://cdn.elifesciences.org/articles/93013/elife-93013-v1.xml https://elifesciences.org/articles/93013 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.5F19A4CA |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.93013 |
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