Academic Journal
The role of microRNAs in ovarian follicle development in zebrafish
| العنوان: | The role of microRNAs in ovarian follicle development in zebrafish |
|---|---|
| المساهمون: | Xu, Wen (author.), Cheng, Hon Ki (thesis advisor.), Chinese University of Hong Kong Graduate School. Division of Biomedical Sciences. (degree granting institution.) |
| سنة النشر: | 2019 |
| المجموعة: | The Chinese University of Hong Kong: CUHK Digital Repository / 香港中文大學數碼典藏 |
| مصطلحات موضوعية: | Zebra danio--Genetics, Graafian follicle, Zebrafish--genetics, Ovarian Follicle--growth & development, QL638.C94 X83 2019eb |
| الوصف: | Ph.D. ; Female fertility is determined by the development of healthy and competent oocytes, which should complete meiosis, could be fertilized and generate healthy embryos. In females, follicles comprised of the central oocytes and surrounding somatic compartments are the functional units of reproductive biology. Bi-directional actions between oocytes and the surrounding follicular cells have been demonstrated to play an essential role in folliculogenesis. microRNAs are small non-coding RNAs which regulate the expression of as many as 30% mammalian protein-coding genes at post-transcriptional level. An increasing number of evidences has indicated that microRNAs are involved in a variety of biological processes as well as developmental and physiological processes. Activities of microRNAs in mouse oocytes and early embryonic development have been shown to be globally suppressed. However, knowledge about the function of microRNAs from follicular cells in folliculogenesis has been limited. ; In the present study, we used BACK recombineering to construct conditional knockout of dgcr8, a gene involved in microRNA biogenesis, in follicular cells. For the construction of dgcr8 cKO zebrafish line, the endogenous dgcr8 was first excised by TALENs technology. A loxP-flanked exogeneous dgcr8 BAC was then integrated into zebrafish genome. When this loxP line was crossed with a Cre line, in which the expression of Cre was specifically driven by an ovarian follicular gene, fshr, dgcr8 was specifically deleted in ovarian follicular cells. Results of genotyping indicated that only ovaries and follicular cells, but not other tissues and oocytes, were observed with dgcr8 deletion. Results of real-time PCR showed that the expression of dgcr8 was significantly decreased in of dgcr8 cKO female follicular cells which resulted in an accumulated expression of primary microRNAs and a decreased expression of mature microRNAs. Both up-regulated and down-regulated expression of genes related to estradiol synthesis, were observed in dgcr8 ... |
| نوع الوثيقة: | text |
| وصف الملف: | electronic resource; remote; 1 online resource (xv, 112 leaves) : illustrations (some color); computer; online resource |
| اللغة: | English Chinese |
| Relation: | cuhk:2399201; local: ETD920201418; local: AAI27784047; local: 991039874568003407; https://julac.hosted.exlibrisgroup.com/primo-explore/search?query=addsrcrid,exact,991039874568003407,AND&tab=default_tab&search_scope=All&vid=CUHK&mode=advanced&lang=en_US; https://repository.lib.cuhk.edu.hk/en/item/cuhk-2399201 |
| الاتاحة: | https://julac.hosted.exlibrisgroup.com/primo-explore/search?query=addsrcrid,exact,991039874568003407,AND&tab=default_tab&search_scope=All&vid=CUHK&mode=advanced&lang=en_US https://repository.lib.cuhk.edu.hk/en/item/cuhk-2399201 |
| Rights: | Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-NoDerivatives 4.0 International" License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
| رقم الانضمام: | edsbas.5DF7ECF8 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |