Academic Journal
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host
| العنوان: | Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
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| المؤلفون: | Mesa-Arango, Ana, Rueda, Cristina, Román, Elvira, Quintin, Jessica, Terrón, María, Luque, Daniel, Netea, Mihai, Pla, Jesus, Zaragoza, Oscar |
| المساهمون: | Instituto de Salud Carlos III Madrid (ISC), Universidad de Antioquia = University of Antioquia Medellín, Colombia, Universidad Complutense de Madrid = Complutense University of Madrid Madrid (UCM), Radboud University Medical Center Nijmegen, A. C. Mesa-Arango has been supported by fellowships from Fundación Carolina and Instituto de Salud Carlos III. C. Rueda has a Sara Borrell contract from the Fondo de Investigaciones Sanitarias (reference no. CD11/00110). |
| المصدر: | ISSN: 0066-4804. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2016 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | MESH: Amphotericin B, MESH: Animals, MESH: Immunity, Innate, MESH: Larva, MESH: Leukocytes, Mononuclear, MESH: Melanins, MESH: Microbial Sensitivity Tests, MESH: Mitogen-Activated Protein Kinases, MESH: Moths, MESH: beta-Glucans, MESH: Antifungal Agents, MESH: Candida tropicalis, MESH: Cell Wall, MESH: Chitin, MESH: Congo Red, MESH: Drug Resistance, Fungal, MESH: Host-Pathogen Interactions, MESH: Humans, [SDV]Life Sciences [q-bio], [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology |
| الوصف: | International audience ; We have morphologically characterized Candida tropicalis isolates resistant to amphotericin B (AmB). These isolates present an enlarged cell wall compared to isolates of regular susceptibility. This correlated with higher levels of β-1,3-glucan in the cell wall but not with detectable changes in chitin content. In line with this, AmB-resistant strains showed reduced susceptibility to Congo red. Moreover, mitogen-activated protein kinases (MAPKs) involved in cell integrity were already activated during regular growth in these strains. Finally, we investigated the response elicited by human blood cells and found that AmB-resistant strains induced a stronger proinflammatory response than susceptible strains. In agreement, AmB-resistant strains also induced stronger melanization of Galleria mellonella larvae, indicating that the effect of alterations of the cell wall on the immune response is conserved in different types of hosts. Our results suggest that resistance to AmB is associated with pleiotropic mechanisms that might have important consequences, not only for the efficacy of the treatment but also for the immune response elicited by the host. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/26833156; pasteur-02873961; https://hal-pasteur.archives-ouvertes.fr/pasteur-02873961; PUBMED: 26833156; PUBMEDCENTRAL: PMC4808153 |
| DOI: | 10.1128/AAC.02681-15 |
| الاتاحة: | https://hal-pasteur.archives-ouvertes.fr/pasteur-02873961 https://doi.org/10.1128/AAC.02681-15 |
| رقم الانضمام: | edsbas.5DE3DC57 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/AAC.02681-15 |
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