Academic Journal
Genome-wide RNAi screen for synthetic lethal interactions with the C. elegans kinesin-5 homolog BMK-1
| العنوان: | Genome-wide RNAi screen for synthetic lethal interactions with the C. elegans kinesin-5 homolog BMK-1 |
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| المؤلفون: | Maia, AF, Tanenbaum, ME, Galli, M, Lelieveld, D, Egan, DA, Gassmann, R, Sunkel, CE, den, Heuvel, S, Medema, RH |
| المساهمون: | Instituto de Investigação e Inovação em Saúde |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2015 |
| المجموعة: | Repositório Aberto da Universidade do Porto |
| مصطلحات موضوعية: | Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins/genetics, Caenorhabditis elegans Proteins/metabolism, Genes, Lethal, Genome, Helminth, Kinesin/genetics, Kinesin/metabolism, Microtubule-Associated Proteins/genetics, Microtubule-Associated Proteins/metabolism, RNA Interference, Signal Transduction, Spindle Apparatus |
| الوصف: | Kinesins are a superfamily of microtubule-based molecular motors that perform various transport needs and have essential roles in cell division. Among these, the kinesin-5 family has been shown to play a major role in the formation and maintenance of the bipolar mitotic spindle. Moreover, recent work suggests that kinesin-5 motors may have additional roles. In contrast to most model organisms, the sole kinesin-5 gene in Caenorhabditis elegans, bmk-1, is not required for successful mitosis and animals lacking bmk-1 are viable and fertile. To gain insight into factors that may act redundantly with BMK-1 in spindle assembly and to identify possible additional cellular pathways involving BMK-1, we performed a synthetic lethal screen using the bmk-1 deletion allele ok391. We successfully knocked down 82% of the C. elegans genome using RNAi and assayed viability in bmk-1(ok391) and wild type strains using an automated high-throughput approach based on fluorescence microscopy. The dataset includes a final list of 37 synthetic lethal interactions whose further study is likely to provide insight into kinesin-5 function. ; We thank the members of the Medema, Kops, Lens, Boxem, The, van den Heuvel, Carvalho and Gassmann laboratories for helpful discussion. To Belen Fernandez-Garcia for helping on hit picking from the genome-wide library. To Oliver Pelz for help with web cellHTS2 application during data analysis. A.F. Maia is a FCT-Fundacao para a Ciencia e a Tecnologia postdoctoral fellow (SFRH/BPD/71364/2010). The R.H. Medema laboratory was supported by the Netherlands Organization for Scientific Research (ZonMw 918.46.616) and the Netherlands Genomics Initiative. S. van den Heuvel received funding from the Netherlands Organisation for Scientific Research (NWO), Chemical Sciences (CW ECHO project 711.011.010). Work on C.E. Sunkel laboratory was funded by FEDER funds through the Operational Competitiveness Programme-COMPETE and by National Funds through FCT-Fundacao para a Ciencia e a Tecnologia under the project ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2052-4463 |
| Relation: | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F71364%2F2010/PT; info:eu-repo/grantAgreement/FCT/COMPETE/120366/PT; Scientific data, 2:150020; https://www.nature.com/articles/sdata201520; http://hdl.handle.net/10216/114508 |
| DOI: | 10.1038/sdata.2015.20 |
| الاتاحة: | http://hdl.handle.net/10216/114508 https://doi.org/10.1038/sdata.2015.20 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.5DCFA4B1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20524463 |
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| DOI: | 10.1038/sdata.2015.20 |