Academic Journal

Identification and characterization of Daldinia eschscholtziiisolated from skin scrapings, nails, and blood

التفاصيل البيبلوغرافية
العنوان: Identification and characterization of Daldinia eschscholtziiisolated from skin scrapings, nails, and blood
المؤلفون: Ng, Kee Peng, Chan, Chai Ling, Yew, Su Mei, Yeo, Siok Koon, Toh, Yue Fen, Looi, Hong Keat, Na, Shiang Ling, Lee, Kok Wei, Yee, Wai-Yan, Kuan, Chee Sian
المساهمون: High Impact Research MoE
المصدر: PeerJ ; volume 4, page e2637 ; ISSN 2167-8359
بيانات النشر: PeerJ
سنة النشر: 2016
المجموعة: PeerJ (E-Journal - via CrossRef)
الوصف: Background Daldinia eschscholtzii is a filamentous wood-inhabiting endophyte commonly found in woody plants. Here, we report the identification and characterization of nine D. eschscholtzii isolates from skin scrapings, nail clippings, and blood. Methods The nine isolates were identified based on colony morphology, light microscopy, and internal transcribed spacer (ITS)-based phylogeny. In vitro antifungal susceptibility of the fungal isolates was evaluated by the Etest to determine the minimum inhibitory concentration (MIC). Results The nine isolates examined were confirmed as D. eschscholtzii . They exhibited typical features of Daldinia sp. on Sabouraud Dextrose Agar, with white felty colonies and black-gray coloration on the reverse side. Septate hyphae, branching conidiophore with conidiogenous cells budding from its terminus, and nodulisporium-like conidiophores were observed under the microscope. Phylogenetic analysis revealed that the nine isolates were clustered within the D. eschscholtzii species complex. All the isolates exhibited low MICs against azole agents (voriconazole, posaconazole, itraconazole, and ketoconazole), as well as amphotericin B, with MIC of less than 1 µg/ml. Discussion Early and definitive identification of D. eschscholtzii is vital to reducing misuse of antimicrobial agents. Detailed morphological and molecular characterization as well as antifungal profiling of D. eschscholtzii provide the basis for future studies on its biology, pathogenicity, and medicinal potential.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.7717/peerj.2637
الاتاحة: http://dx.doi.org/10.7717/peerj.2637
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https://peerj.com/articles/2637.html
Rights: http://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.5D8610B1
قاعدة البيانات: BASE