Academic Journal
Requirement of MMP‐3 in anchorage‐independent growth of oral squamous cell carcinomas
| العنوان: | Requirement of MMP‐3 in anchorage‐independent growth of oral squamous cell carcinomas |
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| المؤلفون: | Liu, Shyun‐Yeu, Liu, Young‐Chau, Huang, Wen‐Tsung, Huang, Guan‐Cheng, Su, Hsiao‐Jing, Lin, Mei‐Huei |
| المصدر: | Journal of Oral Pathology & Medicine ; volume 36, issue 7, page 430-435 ; ISSN 0904-2512 1600-0714 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2007 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background: Matrix metalloproteinase‐3 (MMP‐3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs). Materials and methods: Specimens of seven oral pre‐malignant lesions (OPMLs) and 92 OSCCs were subjected to MMP‐3 detection by RT‐PCR and Western blot. Antisense oligodeoxynucleotides (AODNs) of MMP‐3 were used to transfect OSCC (OECM‐1 and SCC‐9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was subsequently analyzed by XTT and soft‐agar colony assay. Results: MMP‐3 transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs (two of seven, 29%; P = 0.012). Both MMP‐3 transcript and protein levels were significantly higher in OSCC masses than in neighboring tissues ( P < 0.0001 and P = 0.04, respectively). Growth of the three cell lines was not affected, while the colony numbers of OECM‐1 and CE81T/VGH were significantly reduced by the transfection of MMP‐3 AODNs ( P = 0.002 and P = 0.004, respectively). SCC‐9 did not form colonies in soft‐agar/medium. Conclusions: MMP‐3 function may be required in most OSCCs, and it may support the anchorage‐independent growth of both OSCC and esophageal carcinoma. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1600-0714.2007.00524.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1600-0714.2007.00524.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0714.2007.00524.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0714.2007.00524.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.5C434AB7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1600-0714.2007.00524.x |
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