التفاصيل البيبلوغرافية
| العنوان: |
RBD trafficking with CG cargo is localized to acidic compartments and endosomal acidification inhibitors neutralize these endosomes. |
| المؤلفون: |
Chaitra Prabhakara (11114263), Rashmi Godbole (4850548), Parijat Sil (11114266), Sowmya Jahnavi (11114269), Shah-e-Jahan Gulzar (11114272), Thomas S. van Zanten (11114275), Dhruv Sheth (11114278), Neeraja Subhash (11114281), Anchal Chandra (11114284), Akshatha Shivaraj (11114287), Patricia Panikulam (11114290), Ibrahim U (11114293), Vijay Kumar Nuthakki (11114296), Theja Parassini Puthiyapurayil (11114299), Riyaz Ahmed (8191644), Ashaq Hussain Najar (11114302), Sai Manoz Lingamallu (11114305), Snigdhadev Das (11114308), Bhagyashri Mahajan (11114311), Praveen Vemula (10035594), Sandip B. Bharate (1575007), Parvinder Pal Singh (1456687), Ram Vishwakarma (196052), Arjun Guha (525950), Varadharajan Sundaramurthy (5898722), Satyajit Mayor (33444) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Microbiology, Molecular Biology, Pharmacology, Immunology, Cancer, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, pH-dependent endocytic pathway, uptake, inhibition, NH 4 Cl, Niclosamide, ACE 2. Ectopic expression, attenuates Spike-pseudovirus entry, Endosomal acidification inhibitors, CLIC, SARS-CoV -2, SARS-CoV -2 spike protein, BafilomycinA 1, endosomal pH, CG, infection, target SARS-CoV -2 entry, AGS-ACE 2 cells, SARS-CoV -2 entry inhibitor, receptor binding domain |
| الوصف: |
A, B: AGS cells were pulsed with RBD for 30 mins or 3 hours, labelled with lysotracker in the last 15 minutes of pulse and imaged live at high resolution. Images are shown in A and quantification of Manders’ co-occurrence coefficient is shown in B. RBD is colocalized with Lysotracker positive compartments. With increase in time more RBD is associated with Lysostracker (p-value < e-06) and more Lysotracker positive compartments have RBD (p-value < e-06). Each condition has >12 cells. Dashed white line in A represents approximate cell boundary. C: Schematic describing the experimental protocol for estimating the pH of endosomes by ratiometric measurements using pH-sensitive (FITC) and pH-insensitive (TMR) dextran. D-F: AGS cells were pulsed with FITC and TMR dextran for 2 hours, chased for 1 hour with BafA1 200nM/400nM, NH 4 Cl 30mM or control and imaged live. Endosomal pH is increased upon addition of acidification inhibitors (p-values < e-118 for BafA1 200nM, < e-122 for BafA1 400nM, < e-223 for NH 4 Cl). Images along with pH maps are shown in D (and in S4C) and quantification in E (and in S4D). Enlarged regions of pH maps indicated by white boxes are shown in F. Box plot in E represents the distribution of medians of each repeat which is denoted by red dots. Violin plot indicates all the data points from repeats. Colour bar in F corresponds to the estimated endosomal pH. Control 1 is 0.2% DMSO, Control 2 is 0.4% DMSO and Control 3 is 0% DMSO. Number of repeats ≥ 3 for each treatment and each repeat has >80 cells. Scale bar: 20μm (A) and 40μm (D). |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/RBD_trafficking_with_CG_cargo_is_localized_to_acidic_compartments_and_endosomal_acidification_inhibitors_neutralize_these_endosomes_/14962759 |
| DOI: |
10.1371/journal.ppat.1009706.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1009706.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.5BFD90D2 |
| قاعدة البيانات: |
BASE |