التفاصيل البيبلوغرافية
| العنوان: |
Influence of Uranium Concentration and pH on U‑Phosphate Biomineralization by Caulobacter OR37 |
| المؤلفون: |
Keith D. Morrison (5228483), Mavrik Zavarin (17481), Annie B. Kersting (1524613), James D. Begg (1646479), Harris E. Mason (1641886), Enrica Balboni (3630061), Yongqin Jiao (1746865) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Molecular Biology, Biotechnology, Ecology, Immunology, Infectious Diseases, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, 0.126 μ M, Caulobacter, EPA, source, intracellular polyphosphate granules, Microbial phosphatase activity, U-contaminated, Pi, drinking water, formation, pH, U concentrations approach autunite ., Environmental Protection Agency, 37 Uranium contamination, site, remediation, mineral |
| الوصف: |
Uranium contamination of soils and groundwater in the United States represents a significant health risk and will require multiple remediation approaches. Microbial phosphatase activity coupled to the addition of an organic P source has recently been studied as a remediation strategy that provides an extended release of inorganic P (Pi) into U-contaminated sites, resulting in the precipitation of meta -autunite minerals. Previous laboratory- and field-based biomineralization studies have investigated environments with relatively high U concentrations (>20 μM). However, most contaminated sites have much lower U concentrations (<2 μM). The Environmental Protection Agency (EPA) limit for U in drinking water is 0.126 μM. Reaching this regulatory limit becomes challenging as U concentrations approach autunite solubility. We studied the precipitation of U(VI)-phosphate minerals by an environmental isolate of Caulobacter sp. (strain OR37) from an Oak Ridge, Tennessee, U-contaminated site. Abiotic U(VI) solubility experiments reveal that U(VI)-phosphate minerals do not form in the presence of excess Pi (500 μM) when U(VI) concentrations are <1 μM and pH is <5. When OR37 cells are reacted under the same conditions with Pi or glycerol-2-phosphate, U(VI)-phosphate mineral formation was observed, along with the formation of intracellular polyphosphate granules. These results show that bacteria provide supersaturated microenvironments needed for U(VI)-phosphate mineralization while hydrolyzing organic P sources. This provides a pathway to lower U concentrations to below EPA limits for drinking water. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Influence_of_Uranium_Concentration_and_pH_on_U_Phosphate_Biomineralization_by_i_Caulobacter_i_OR37/13619183 |
| DOI: |
10.1021/acs.est.0c05437.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.est.0c05437.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.5BDBAC79 |
| قاعدة البيانات: |
BASE |