التفاصيل البيبلوغرافية
| العنوان: |
Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
| المؤلفون: |
Ágnes Rusznyák, Mercédesz Palicskó, Milo Malanga, Éva Fenyvesi, Lajos Szente, Judit Váradi, Ildikó Bácskay, Miklós Vecsernyés, Katalin Szászné Réti-Nagy, Gábor Vasvári, Ádám Haimhoffer, Ferenc Fenyvesi |
| المصدر: |
Molecules; Volume 27; Issue 5; Pages: 1589 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
HPBCD, RAMEB, endocytosis, lysosomes, autophagy, NF-κB, HeLa |
| جغرافية الموضوع: |
agris |
| الوصف: |
Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Applied Chemistry; https://dx.doi.org/10.3390/molecules27051589 |
| DOI: |
10.3390/molecules27051589 |
| الاتاحة: |
https://doi.org/10.3390/molecules27051589 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.5BBCF2CC |
| قاعدة البيانات: |
BASE |