Academic Journal
Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models
| العنوان: | Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models |
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| المؤلفون: | Wang, Chih-Wen, Chiou, Hsin-Ying Clair, Chen, Szu-Chia, Wu, Da-Wei, Lin, Hung-Hsun, Chen, Huang-Chi, Liao, Wei-Ting, Lin, Ming-Hong, Hung, Chih-Hsing, Kuo, Chao-Hung |
| المصدر: | Frontiers in Immunology ; volume 14 ; ISSN 1664-3224 |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2023 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Introduction Arsenic (As) exposure is associated with lung toxicity and we aim to investigate the effects of arsenic exposure on lung fibrotic changes. Methods Participants (n= 976) enrolled via a general health survey underwent chest low-dose computed tomography (LDCT), spirometry forced expiratory volume in 1 s (FEV1), forced vital capacity (FVC), and urinary arsenic examination during 2016 and 2018. Lung fibrotic changes from LDCT were defined. As LtoL , low arsenic levels in both 2016 and 2018; As LtoH , low arsenic in 2016 but high levels in 2018; As HtoL , high arsenic in 2016 but low levels in 2018; As HtoH , high arsenic levels in both 2016 and 2018. Mice exposed to 0. 0.2mg/L, 2 mg/L, 50 mg/L of sodium arsenite (NaAsO 2 ) through drinking water for 12 weeks and 24 weeks were applied for histological analysis. Cultured lung epithelial cells were exposed to NaAsO 2 and the mesenchymal changes were examined. Results As HtoH increased the risk (OR= 1.65, 95% CI 1.10, 2.49) of Lung fibrotic positive to positive (reference: Lung fibrotic negative to negative ) compared with As LtoL . Moreover, the predicted mean of FVC and FEV1 in As HtoH (−0.09 units, 95% CI: −0.27, −0.09; −0.09 units, 95% CI: −0.17, −0.01) and As LtoH (−0.13 units, 95% CI: −0.30, −0.10; −0.13 units, 95% CI: −0.22, −0.04) was significantly lower than AS LtoL . Significant lung fibrotic changes including the increase of the alveolar septum thickness and collagen fiber deposition were observed upon 2 mg/L NaAsO 2 treatment for 12 weeks, and the damage was dose- and time-dependent. In vitro , sodium arsenite treatment promotes the epithelial-mesenchymal transition (EMT)-like changes of the normal human bronchial epithelial cells, including upregulation of several fibrotic and mesenchymal markers (fibronectin, MMP-2, and Snail) and cell migration. Inhibition of reactive oxygen species (ROS) and MMP-2 impaired the arsenic-induced EMT changes. Administration of a flavonoid, apigenin, inhibited EMT in vitro and pulmonary damages in vivo with the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fimmu.2023.1225348 |
| DOI: | 10.3389/fimmu.2023.1225348/full |
| الاتاحة: | http://dx.doi.org/10.3389/fimmu.2023.1225348 https://www.frontiersin.org/articles/10.3389/fimmu.2023.1225348/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.5BB838B2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2023.1225348 |
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