Academic Journal
Heat selection enables highly scalable methylome profiling in cell-free DNA for noninvasive monitoring of cancer patients
| العنوان: | Heat selection enables highly scalable methylome profiling in cell-free DNA for noninvasive monitoring of cancer patients |
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| المؤلفون: | Cheruba, Elsie, Viswanathan, Ramya, Wong, Pui-Mun, Womersley, Howard John, Han, Shuting, Tay, Brenda, Lau, Yiting, Gan, Anna, Poon, Polly S. Y., Skanderup, Anders, Ng, Sarah B., Chok, Aik Yong, Chong, Dawn Qingqing, Tan, Iain Beehuat, Cheow, Lih Feng |
| المصدر: | Science Advances ; volume 8, issue 36 ; ISSN 2375-2548 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS) |
| سنة النشر: | 2022 |
| الوصف: | Genome-wide analysis of cell-free DNA methylation profile is a promising approach for sensitive and specific detection of many cancers. However, scaling such assays for clinical translation is impractical because of the high cost of whole-genome bisulfite sequencing. We show that the small fraction of GC-rich genome is highly enriched in CpG sites and disproportionately harbors most of the cancer-specific methylation signature. Here, we report on the simple and effective heat enrichment of CpG-rich regions for bisulfite sequencing (Heatrich-BS) platform that allows for focused methylation profiling in these highly informative regions. Our novel method and bioinformatics algorithm enable accurate tumor burden estimation and quantitative tracking of colorectal cancer patient’s response to treatment at much reduced sequencing cost suitable for frequent monitoring. We also show tumor epigenetic subtyping using Heatrich-BS, which could enable patient stratification. Heatrich-BS holds great potential for highly scalable screening and monitoring of cancer using liquid biopsy. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1126/sciadv.abn4030 |
| الاتاحة: | http://dx.doi.org/10.1126/sciadv.abn4030 https://www.science.org/doi/pdf/10.1126/sciadv.abn4030 |
| رقم الانضمام: | edsbas.5B231294 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1126/sciadv.abn4030 |
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