Academic Journal

Genome Survey of Male and Female Spotted Scat (Scatophagus argus)

التفاصيل البيبلوغرافية
العنوان: Genome Survey of Male and Female Spotted Scat (Scatophagus argus)
المؤلفون: Yuanqing Huang, Dongneng Jiang, Ming Li, Umar Farouk Mustapha, Changxu Tian, Huapu Chen, Yang Huang, Siping Deng, Tianli Wu, Chunhua Zhu, Guangli Li
المصدر: Animals; Volume 9; Issue 12; Pages: 1117
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2019
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: genome size, illumina sequencing, Dmrt1, sex determining gene, aquaculture, sex control
جغرافية الموضوع: agris
الوصف: The spotted scat, Scatophagus argus, is a species of fish that is widely propagated within the Chinese aquaculture industry and therefore has significant economic value. Despite this, studies of its genome are severely lacking. In the present study, a genomic survey of S. argus was conducted using next-generation sequencing (NGS). In total, 55.699 GB (female) and 51.047 GB (male) of high-quality sequence data were obtained. Genome sizes were estimated to be 598.73 (female) and 597.60 (male) Mbp. The sequence repeat ratios were calculated to be 27.06% (female) and 26.99% (male). Heterozygosity ratios were 0.37% for females and 0.38% for males. Reads were assembled into 444,961 (female) and 453,459 (male) contigs with N50 lengths of 5,747 and 5,745 bp for females and males, respectively. The average guanine-cytosine (GC) content of the female genome was 41.78%, and 41.82% for the male. A total of 42,869 (female) and 43,283 (male) genes were annotated to the non-redundant (NR) and SwissProt databases. The female and male genomes contained 66.6% and 67.8% BUSCO core genes, respectively. Dinucleotide repeats were the dominant form of simple sequence repeats (SSR) observed in females (68.69%) and males (68.56%). Additionally, gene fragments of Dmrt1 were only observed in the male genome. This is the first report of a genome-wide characterization of S. argus.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
Relation: Animal Genetics and Genomics; https://dx.doi.org/10.3390/ani9121117
DOI: 10.3390/ani9121117
الاتاحة: https://doi.org/10.3390/ani9121117
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.5AF18D98
قاعدة البيانات: BASE