Academic Journal
Rapid isolation of promoter sequences
| العنوان: | Rapid isolation of promoter sequences |
|---|---|
| المؤلفون: | R. Terauchi, Æ G. Kahl, Of Pal, Key Tail-pcr, Æ Promoter, Æ Dioscorea, Æ Pal |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://download.bioon.com.cn/view/upload/month_1004/20100415_9a3d7eed95fed4d5c376b5l2zr9iuwB6.attach.pdf. |
| سنة النشر: | 1999 |
| المجموعة: | CiteSeerX |
| الوصف: | Using a modified TAIL-PCR technique, the 5¢-flanking regions of the phenylalanine ammonia lyase (Pal) genes of a yam species, Dioscorea bulbifera, and the phosphoglucose isomerase (Pgi) gene of D. tokoro were successfully isolated. Two novel modifications of the TAIL-PCR procedure introduced here, namely (1) the use of a battery of random 10-mers (RAPD primers) as short arbitrary primers, and (2) the use of a total of five nested, gene-specific primers, allow the rapid isolation of the 5¢-flanking region of any gene from organisms with large genomes. Isolated 5¢-flanking regions were fused to the gus gene, and tested for transient expression in to-bacco BY2 cells. All the isolated 5¢-flanking regions were shown to drive reporter gene expression. Three Pal promoters responded to salicylic acid, presumably as a result of the binding of a MYB transcriptional activator to the multiple MREs (Myb Recognition Elements) present in these regions. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.469.4766; http://download.bioon.com.cn/view/upload/month_1004/20100415_9a3d7eed95fed4d5c376b5l2zr9iuwB6.attach.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.469.4766 http://download.bioon.com.cn/view/upload/month_1004/20100415_9a3d7eed95fed4d5c376b5l2zr9iuwB6.attach.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.5AC5018F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |