Academic Journal
A Polylactide-Based Micellar Adjuvant Improves the Intensity and Quality of Immune Response
| العنوان: | A Polylactide-Based Micellar Adjuvant Improves the Intensity and Quality of Immune Response |
|---|---|
| المؤلفون: | Lamrayah, Myriam, Phelip, Capucine, Coiffier, Céline, Lacroix, Céline, Willemin, Thibaut, Trimaille, Thomas, Verrier, Bernard |
| المساهمون: | Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305 (LBTI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Ingénierie des Matériaux Polymères (IMP), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Sidaction grant (grant number 11623), ANRS PhD funding (grant number HIVERA-002), CNRS, ANR-16-ENM2-0001,FluNanoAir,Design of Human Influenza vaccines using multifunctional micelles harnessing innate immunity(2016) |
| المصدر: | ISSN: 1999-4923 ; Pharmaceutics ; https://hal.science/hal-04145333 ; Pharmaceutics, 2022, 14 (1), pp.107. ⟨10.3390/pharmaceutics14010107⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Université Jean Monnet – Saint-Etienne: HAL |
| مصطلحات موضوعية: | micelles, vaccine delivery, immunization, adjuvants, nanoparticles, biodistribution, p24, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology |
| الوصف: | International audience ; Micelles from amphiphilic polylactide-block-poly(N-acryloxysuccinimide-co-N-vinylpyrrolidone) (PLA-b-P(NAS-co-NVP)) block copolymers of 105 nm in size were characterized and evaluated in a vaccine context. The micelles were non-toxic in vitro (both in dendritic cells and HeLa cells). In vitro fluorescence experiments combined with in vivo fluorescence tomography imaging, through micelle loading with the DiR near infrared probe, suggested an efficient uptake of the micelles by the immune cells. The antigenic protein p24 of the HIV-1 was successfully coupled on the micelles using the reactive N-succinimidyl ester groups on the micelle corona, as shown by SDS-PAGE analyses. The antigenicity of the coupled antigen was preserved and even improved, as assessed by the immuno-enzymatic (ELISA) test. Then, the performances of the micelles in immunization were investigated and compared to different p24-coated PLA nanoparticles, as well as Alum and MF59 gold standards, following a standardized HIV-1 immunization protocol in mice. The humoral response intensity (IgG titers) was substantially similar between the PLA micelles and all other adjuvants over an extended time range (one year). More interestingly, this immune response induced by PLA micelles was qualitatively higher than the gold standards and PLA nanoparticles analogs, expressed through an increasing avidity index over time (>60% at day 365). Taken together, these results demonstrate the potential of such small-sized micellar systems for vaccine delivery. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04145333; https://hal.science/hal-04145333; https://hal.science/hal-04145333/document; https://hal.science/hal-04145333/file/pharmaceutics-14-00107.pdf |
| DOI: | 10.3390/pharmaceutics14010107 |
| الاتاحة: | https://hal.science/hal-04145333 https://hal.science/hal-04145333/document https://hal.science/hal-04145333/file/pharmaceutics-14-00107.pdf https://doi.org/10.3390/pharmaceutics14010107 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5A1E7B2E |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/pharmaceutics14010107 |
|---|