التفاصيل البيبلوغرافية
| العنوان: |
Effect of guanosine on viral progeny production of Vesicular Stomatitis Virus (VSV), Foot-and-mouth Disease Virus (FMDV) and Lymphocytic Choriomeningitis Virus (LCMV). |
| المؤلفون: |
Rosario Sabariegos (12008820), Ana María Ortega-Prieto (12008823), Luis Díaz-Martínez (12008826), Ana Grande-Pérez (446896), Carlos García Crespo (12008829), Isabel Gallego (3226725), Ana I. de Ávila (3226722), Laura Albentosa-González (12008832), María Eugenia Soria (5814956), Pablo Gastaminza (2855585), Esteban Domingo (86438), Celia Perales (227281), Antonio Mas (225243) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Microbiology, Cell Biology, Molecular Biology, Cancer, Mental Health, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, ndp balance appears, increases indel frequencies, human hepatoma cells, hepatitis c virus, de novo <, ntps ), leading, inhibits hcv replication, div >< p, vitro <, viral rna, viral progeny, template switching, standard nucleosides, specific infectivity, significant increase, rna synthesis, proper ntp, polymerase activity |
| الوصف: |
BHK21c2 cells were seeded and 16 hours prior to infection cells were untreated or treated with 800 μM of guanosine. Cells were infected with two different m.o.i. (0.05 or 0.0005), supernatants were collected at different times after infection (0-, 2-, 5-, 8- and 24-hours post-infection) and viral progeny production was analyzed. The m.o.i. and the treatment used are indicated in the upper box. The viral titer expressed as log10 pfu/ml is indicated in the ordinate. The time post-infection is indicated in the abscissa expressed in hours. ( A ) Effect of the addition of 800 μM of guanosine on VSV infectious progeny production. The differences between the slope of the treated and untreated has been calculated using an ANCOVA test in GraphPad (m.o.i. 0.05 p-value = 0.86; m.o.i. 0.0005 p-value = 0.62). ( B, C ) Same as A but the virus used are FMDV (B) and LCMV (C). Statistical differences between slopes were not found for FMDV: (m.o.i. 0.05 p-value = 0.45; m.o.i. 0.0005 p-value = 0.26) and for LCMV: (m.o.i. 0.05 p-value = 0.58; m.o.i. 0.0005 p-value = 0.79). Titrations were carried out in triplicate. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Effect_of_guanosine_on_viral_progeny_production_of_Vesicular_Stomatitis_Virus_VSV_Foot-and-mouth_Disease_Virus_FMDV_and_Lymphocytic_Choriomeningitis_Virus_LCMV_/19078641 |
| DOI: |
10.1371/journal.ppat.1010210.s002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1010210.s002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.59FABA4A |
| قاعدة البيانات: |
BASE |