Academic Journal
Real-Time Quantitative PCR Assay for Monitoring of Nervous Necrosis Virus Infection in Grouper Aquaculture
| العنوان: | Real-Time Quantitative PCR Assay for Monitoring of Nervous Necrosis Virus Infection in Grouper Aquaculture |
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| المؤلفون: | Kuo, Hsiao-Che, Wang, Ting-Yu, Chen, Peng-Peng, Chen, Young-Mao, Chuang, Hui-Ching, Chen, Tzong-Yueh |
| المصدر: | Journal of Clinical Microbiology ; volume 49, issue 3, page 1090-1096 ; ISSN 0095-1137 1098-660X |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2011 |
| الوصف: | Viral nervous necrosis caused by nervous necrosis virus (NNV) exacts a high mortality and results in huge economic losses in grouper aquaculture in Taiwan. The present study developed a real-time quantitative PCR (qPCR) method for NNV monitoring. The assay showed a strong linear correlation ( r 2 = 0.99) between threshold cycle ( C T ) and RNA quantities, which allowed identification of infected groupers by the C T value and could be exploited to warn of NNV infection prior to an outbreak in grouper fish farms. Real-time qPCR also confirmed the copious content of NNV in grouper fin, similar to that in primary tissues; the result was verified by using in situ reverse transcription-PCR (RT-PCR). This indicated that grouper fin was a suitable sample for NNV detection, in a manner that could be relatively benign to the fish. The rapid spread of NNV infection to the entire population of affected farms was evident. The developed real-time qPCR method is rapid, highly sensitive, and applicable to routine high-throughput detection of large numbers of samples and has potential as a suitable tool for diagnostic, epidemiological, and genetic studies of grouper aquaculture. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jcm.01016-10 |
| DOI: | 10.1128/JCM.01016-10 |
| الاتاحة: | http://dx.doi.org/10.1128/jcm.01016-10 https://journals.asm.org/doi/pdf/10.1128/JCM.01016-10 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.59E94685 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jcm.01016-10 |
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