التفاصيل البيبلوغرافية
| العنوان: |
Cleavage of Relish in the H2Av 810 mutant. |
| المؤلفون: |
Ruijuan Tang (11250680), Wuren Huang (3493211), Jingmin Guan (107503), Qiuning Liu (5728409), Brenda T. Beerntsen (11250683), Erjun Ling (142303) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Cell Biology, Immunology, Science Policy, Infectious Diseases, Biological Sciences not elsewhere classified, activation, E 3 component, germ-free Drosophila larvae, Relish SUMOylation Insects, IMD-dependent AMP production, Drosophila H 2Av, NF -κB transcription factor Relish, acid K 823. Loss, transcription factor Relish, IMD pathway, histone variant H 2Av upregulate IM., H 2Av, IMD-dependent AMP gene induction |
| الوصف: |
(A, B) qPCR analysis of Relish in the fat bodies of larvae conventionally reared (A) or germ-free (B) before the wandering stage. (C) Western blotting shows Relish and Rel-68 in w 1118 (received Ecc15 challenge or not as a positive or negative control) and H2Av 810 mutants (without Ecc15 challenge). Polyclonal antiserum against Drosophila Relish [ 40 ] was used for the Western blotting assay and active Rel-68 was detected in H2Av 810 . The arrowhead and arrow point to Relish (Rel) and Rel-68 respectively. (D) Commercial antibody against Drosophila Relish (Abin1111036, RayBiotech 130–10080) was applied for immuno-staining fat bodies dissected from w 1118 and Rel E20 received immune challenged according to the published papers [ 41 , 42 ]. Using this commercial antibody, we detected Relish signal in nuclei of fat bodies of w 1118 but not Rel E20 mutant larvae. (E) Immuno-staining to show the distribution of Relish in fat body cells and Rel-68 in nuclei of larval fat bodies of H2Av 810 mutant. The arrows indicate cells with Relish signal inside nuclei. (F-G) Quantification of Relish in whole fat body cells (F) and Rel-68 in nuclei (G). The amount of Rel or Rel-68 produced from fat bodies of w 1118 is equal to 1 for quantification. Two-tailed Student’s t-test was performed. Mean values are presented ±SE. **p < 0.01, ***p < 0.001, and ****p < 0.0001. Bar: 20 μm. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Cleavage_of_Relish_in_the_i_H2Av_i_sup_i_810_i_sup_mutant_/15137237 |
| DOI: |
10.1371/journal.pgen.1009718.s007 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009718.s007 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.59CE2E23 |
| قاعدة البيانات: |
BASE |