Academic Journal
Stroma-rich co-culture multicellular tumor spheroids as a tool for photoactive drugs screening
| العنوان: | Stroma-rich co-culture multicellular tumor spheroids as a tool for photoactive drugs screening |
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| المؤلفون: | Yakavets, Ilya, Jenard, Samuel, François, Aurélie, Maklygina, Yulia, Loschenov, Victor, Lassalle, Henri-Pierre, Dolivet, Gilles, Bezdetnaya, Lina |
| المساهمون: | Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut de Cancérologie de Lorraine - Alexis Vautrin Nancy (UNICANCER/ICL), UNICANCER, Institut Jules Bordet Bruxelles, Faculté de Médecine Bruxelles (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), A. M. Prokhorov General Physics Institute (GPI), Russian Academy of Sciences Moscow (RAS), The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) Moscow, Russia |
| المصدر: | ISSN: 2077-0383 ; Journal of Clinical Medicine ; https://hal.science/hal-02418797 ; Journal of Clinical Medicine, 2019, 8 (10), pp.1686. ⟨10.3390/jcm8101686⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2019 |
| المجموعة: | Université de Lorraine: HAL |
| مصطلحات موضوعية: | photodynamic therapy, temoporfin, head and neck squamous carcinoma, multicellular tumor spheroids, cancer-associated fibroblasts, drug penetration, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology |
| الوصف: | International audience ; Conventional 3D multicellular tumor spheroids of head and neck squamous cell carcinoma (HNSCC) consisting exclusively of cancer cells have some limitations. They are compact cell aggregates that do not interact with their extracellular milieu, thus suffering from both insufficient extracellular matrix (ECM) deposition and absence of different types of stromal cells. In order to better mimic in vivo HNSCC tumor microenvironment, we have constructed a 3D stroma-rich in vitro model of HNSCC, using cancer-associated MeWo skin fibroblasts and FaDu pharynx squamous cell carcinoma. The expression of stromal components in heterospheroids was confirmed by immunochemical staining. The generated co-culture FaDu/MeWo spheroids were applied to study penetration, distribution and antitumor efficacy of photoactive drugs such as Temoporfin and Chlorin e6 used in the photodynamic therapy flow cytometry and fluorescence microscopy techniques. We also investigated the distribution of photodiagnostic agent Indocyanine Green. We demonstrated that the presence of stroma influences the behavior of photoactive drugs in different ways: (i) No effect on Indocyanine Green distribution; (ii) lower accumulation of Chlorin e6; (iii) better penetration and PDT efficiency of Temoporfin. Overall, the developed stroma-rich spheroids enlarge the arsenal of in vitro pre-clinical models for high-throughput screening of anti-cancer drugs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-02418797; https://hal.science/hal-02418797; https://hal.science/hal-02418797/document; https://hal.science/hal-02418797/file/jcm-08-01686-v2.pdf; PUBMEDCENTRAL: PMC6832590 |
| DOI: | 10.3390/jcm8101686 |
| الاتاحة: | https://hal.science/hal-02418797 https://hal.science/hal-02418797/document https://hal.science/hal-02418797/file/jcm-08-01686-v2.pdf https://doi.org/10.3390/jcm8101686 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5960A77D |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/jcm8101686 |
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