Academic Journal
Evidence for the control of aggrecanases by insulin and glucose in alzheimer’s disease [Alzheimer hastalığında insülin ve glukoz ile agrekanazların kontrolü üzerine kanıtlar]
| العنوان: | Evidence for the control of aggrecanases by insulin and glucose in alzheimer’s disease [Alzheimer hastalığında insülin ve glukoz ile agrekanazların kontrolü üzerine kanıtlar] |
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| المؤلفون: | Akyol S., Ugurcu V., Cakmak O., Altuntas A., Yukselten Y., Akyol O., Sunguroglu A. |
| بيانات النشر: | Cukurova Univ Tip Fakultesi Psikiyatri Anabilim Dali |
| سنة النشر: | 2014 |
| المجموعة: | Kütahya Dumlupınar University Institutional Repository |
| مصطلحات موضوعية: | ADAMTS5, Alzheimer’s disease, Diabetes, Hyperglycemia, IL-33, Matrix degradation, NF?B |
| الوصف: | Objective: Alzheimer’s disease (AD) is a progressive and irreversible central nervous system disease, which slowly destroys cognitive skills and memory, and eventually even the ability to handle the simplest tasks. The initiation and progression of AD is a poorly understood complex process. Here, we have investigated possible biological mechanisms that could be responsible for the increased risk for diminished brain function associated with diabetes in AD.Method: The U87 cell line (human primary glioblastoma cell line) was cultured in Dulbecco’s modified Eagle’s medium. Cells were incubated with insulin (10 µg/ml), low glucose (11 mM, 2 mg/ml) and high glucose (55 mM, 10 mg/ml) for 48 hours. Cells were harvested and protein isolations were performed. Primary anti-ADAMTS5, anti- IL-33, anti-NF?B, and anti-GAPDH antibodies were used to detect corresponding proteins and to measure band densities in Western membranes using a specific program.Results: Western blot analysis showed ADAMTS5 protein decreases in insulin-applied U87 cells. High glucose application led to a notable increase in ADAMTS5 levels in cells, while low glucose application caused a moderate increase in ADAMTS5 levels. An apparent induction of IL-33 protein was observed in high glucose-applied cells, while a moderate decrease was noted in the low-glucose applied group. Insulin administration led to a decrease in IL-33 levels. Immunoreaction of NF?B with corresponding antibody was found to be sharply decreased in insulin-applied cells while low and high glucose application led to a moderate decrease in NF?B.Conclusion: This is the first reported study that has investigated both aggrecanases and inflammation mediators in the same experimental setup with U87 cells and interpreted the results in the various aspects of AD pathophysiology related to diabetes and hyperglycemia. Our findings suggest that insulin and glucose may have important functions in the synthesis of ADAMTS, IL-33, and NF?B through undefined mechanism(s). Further investigations dealing ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1017-7833 |
| Relation: | Klinik Psikofarmakoloji Bulteni; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://dx.doi.org/10.5455/bcp.20140905124459; https://hdl.handle.net/20.500.12438/4809; 24; 323; 332 |
| DOI: | 10.5455/bcp.20140905124459 |
| الاتاحة: | https://hdl.handle.net/20.500.12438/4809 https://doi.org/10.5455/bcp.20140905124459 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.59041A9B |
| قاعدة البيانات: | BASE |
| تدمد: | 10177833 |
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| DOI: | 10.5455/bcp.20140905124459 |