Academic Journal
Replication Fork Breakage and Restart in Escherichia coli
| العنوان: | Replication Fork Breakage and Restart in Escherichia coli |
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| المؤلفون: | Michel, Bénédicte, Sinha, Anurag K., Leach, David R. F. |
| المساهمون: | Stabilité de l’ADN bactérien (STABAC), Département Biologie des Génomes (DBG), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), University of Edinburgh (Edin.) |
| المصدر: | ISSN: 1092-2172. |
| بيانات النشر: | CCSD American Society for Microbiology |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | DBG, bacterial-dna replication, cell-division, chromosome dimer resolution, chromosome terminus, double-strand break, double-strand breaks, holliday-junctions, homologous recombination, in-vivo, lagging-strand, PriA, RecA, RecBC, RecBCD, recbcd enzyme, RecG, recombination, recombination-deficient strains, replication fork reversal, replication restart, RuvAB, STABAC, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; In all organisms, replication impairments are an important source of genome rearrangements, mainly because of the formation of double-stranded DNA (dsDNA) ends at inactivated replication forks. Three reactions for the formation of dsDNA ends at replication forks were originally described for Escherichia coli and became seminal models for all organisms: the encounter of replication forks with preexisting single-stranded DNA (ssDNA) interruptions, replication fork reversal, and head-to-tail collisions of successive replication rounds. Here, we first review the experimental evidence that now allows us to know when, where, and how these three different reactions occur in E. coli. Next, we recall our recent studies showing that in wild-type E. coli, spontaneous replication fork breakage occurs in 18% of cells at each generation. We propose that it results from the replication of preexisting nicks or gaps, since it does not involve replication fork reversal or head-to-tail fork collisions. In the recB mutant, deficient for double-strand break (DSB) repair, fork breakage triggers DSBs in the chromosome terminus during cell division, a reaction that is heritable for several generations. Finally, we recapitulate several observations suggesting that restart from intact inactivated replication forks and restart from recombination intermediates require different sets of enzymatic activities. The finding that 18% of cells suffer replication fork breakage suggests that DNA remains intact at most inactivated forks. Similarly, only 18% of cells need the helicase loader for replication restart, which leads us to speculate that the replicative helicase remains on DNA at intact inactivated replication forks and is reactivated by the replication restart proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/29898897; PUBMED: 29898897 |
| DOI: | 10.1128/MMBR.00013-18 |
| الاتاحة: | https://hal.science/hal-02182305 https://doi.org/10.1128/MMBR.00013-18 |
| رقم الانضمام: | edsbas.58E56500 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/MMBR.00013-18 |
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