Academic Journal
The use of the Dhcr7 knockout mouse to accurately determine the origin of fetal sterols
| العنوان: | The use of the Dhcr7 knockout mouse to accurately determine the origin of fetal sterols |
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| المؤلفون: | G.S. Tint, Hongwei Yu, Quan Shang, Guorong Xu, Shailendra B. Patel |
| المصدر: | Journal of Lipid Research, Vol 47, Iss 7, Pp 1535-1541 (2006) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2006 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Smith-Lemli-Opitz syndrome, desmosterol, sterol C24,25-reductase, cholesterol 24-hydroxylase, 7-dehydrocholesterol, 7-dehydrodesmosterol, Biochemistry, QD415-436 |
| الوصف: | Mice with a targeted mutation of 3β-hydroxysterol Δ7-reductase (Dhcr7) that cannot convert 7-dehydrocholesterol to cholesterol were used to identify the origin of fetal sterols. Because their heterozygous mothers synthesize cholesterol normally, virtually all sterols found in a Dhcr7 knockout fetus having a Δ7 or a Δ8 double bond must have been synthesized by the fetus itself but any cholesterol had to have come from the mother. Early in gestation, most fetal sterols were of maternal origin, but at approximately E13–14, in situ synthesis became increasingly important, and by birth, 55–60% of liver and lung sterols had been made by the fetus. In contrast, at E10–11, upon formation of the blood-brain barrier, the brain rapidly became the source of almost all of its own sterols (90% at birth). New, rapid, de novo sterol synthesis in brain was confirmed by the observation that concentrations of C24,25-unsaturated sterols were low in the brains of all very young fetuses but increased rapidly beginning at approximately E11–12. Reduced activity of sterol C24,25-reductase (Dhcr24) in brain, suggested by the abundance of C24,25-unsaturated compounds, seems to be the result of suppressed Dhcr24 expression. The early fetal brain also appears to conserve cholesterol by keeping cholesterol 24-hydroxylase expression low until approximately E18. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-02-222752-4 0-02-222752-0 |
| تدمد: | 0022-2275 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0022227520331989; https://doaj.org/toc/0022-2275; https://doaj.org/article/e41e1b5696a94859ba5b0622a12ea980 |
| DOI: | 10.1194/jlr.M600141-JLR200 |
| الاتاحة: | https://doi.org/10.1194/jlr.M600141-JLR200 https://doaj.org/article/e41e1b5696a94859ba5b0622a12ea980 |
| رقم الانضمام: | edsbas.58E0D42D |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9780022227524 0022227520 |
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| تدمد: | 00222275 |
| DOI: | 10.1194/jlr.M600141-JLR200 |