التفاصيل البيبلوغرافية
| العنوان: |
Dynamical thermal dose models and dose time-profile effects |
| المؤلفون: |
Ladjimi, M. T., Labavic, Darka, Guilbert, Marie, Anquez, Francois, Pruvost, Alexandra, Courtade, Emmanuel, Pfeuty, Benjamin, Thommen, Quentin |
| المساهمون: |
Université de Lille, CNRS |
| بيانات النشر: |
Informa UK Limited |
| سنة النشر: |
2020 |
| المجموعة: |
LillOA (Lille Open Archive - Université de Lille) |
| الوصف: |
Introduction: Models of dose-effect relationships seek systematic and predictive descriptions of how cell survival depends on the level and duration of the stressor. The CEM43 thermal dose model has been empirically derived more than thirty years ago and still serves as a benchmark for hyperthermia protocols despitethe advent of regulatory network models. Objective: In this paper, we propose and realize a simple experimental test to assess whether mech- anistic models can prove more reliable indicators for some protocols. We define two time-asymmetric hyperthermia profiles, faster rise than decay or slower rise than decay, for which the CEM43 model predicts the same survival while a regulatory network model predicts significant differences. Materials: Experimental data (both control 37 C and hyperthermia assays) were collected from dupli- cate HeLa cell cultures. Cells were imaged before and 24, 48 and 72 h after the hyperthermia assay double-stained with fluorescein-5-isothiocyanate (FITC)-labeled annexin V and propidium iodide for detecting cell death. Results: Survival experiments of HeLa cells show that a fast temperature rise followed by a slow decay can be twice more lethal than the opposite, consistently with the prediction of the network model. Conclusions: Using a model reduction approach, we obtained a simple nonlinear dynamic equation that identifies the limited repair capacity as the main factor underlying the dose-asymmetry effect and that could be useful for refining thermal doses for dynamic protocols. ; 36;1 |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/octet-stream |
| اللغة: |
unknown |
| Relation: |
International Journal of Hyperthermia; http://hdl.handle.net/20.500.12210/20208.2 |
| الاتاحة: |
https://hdl.handle.net/20.500.12210/20208.2 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.58DD3946 |
| قاعدة البيانات: |
BASE |