Academic Journal
Flexible Quality Control for Protein Turnover Rates Using d2ome
| العنوان: | Flexible Quality Control for Protein Turnover Rates Using d2ome |
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| المؤلفون: | Henock M. Deberneh, Rovshan G. Sadygov |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 21, p 15553 (2023) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | protein turnover, heavy water metabolic labeling, label incorporation, isotope profiles, retention time alignment, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Bioinformatics tools are used to estimate in vivo protein turnover rates from the LC-MS data of heavy water labeled samples in high throughput. The quantification includes peak detection and integration in the LC-MS domain of complex input data of the mammalian proteome, which requires the integration of results from different experiments. The existing software tools for the estimation of turnover rate use predefined, built-in, stringent filtering criteria to select well-fitted peptides and determine turnover rates for proteins. The flexible control of filtering and quality measures will help to reduce the effects of fluctuations and interferences to the signals from target peptides while retaining an adequate number of peptides. This work describes an approach for flexible error control and filtering measures implemented in the computational tool d2ome for automating protein turnover rates. The error control measures (based on spectral properties and signal features) reduced the standard deviation and tightened the confidence intervals of the estimated turnover rates. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/21/15553; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/7a47bd3f3b9f426092fb524d6dbc02a5 |
| DOI: | 10.3390/ijms242115553 |
| الاتاحة: | https://doi.org/10.3390/ijms242115553 https://doaj.org/article/7a47bd3f3b9f426092fb524d6dbc02a5 |
| رقم الانضمام: | edsbas.58DBB927 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms242115553 |