Academic Journal
Outlining cell interaction and inflammatory cytokines on UV-photofunctionalized mixed-phase TiO2 thin film
| العنوان: | Outlining cell interaction and inflammatory cytokines on UV-photofunctionalized mixed-phase TiO2 thin film |
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| المؤلفون: | Pantaroto, Heloisa Navarro, de Almeida, Amanda B., Gomes, Orisson P., Matos, Adaias O., Landers, Richard, Casarin, Renato Corrêa V., da Silva, José Humberto D., Nociti, Francisco H., Barão, Valentim A.R. |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2021 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Cytokines, Osteoblasts, Sputtering, Titanium, Titanium oxide, Ultraviolet therapy |
| الوصف: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ; CAPES: 001 ; Processo FAPESP: 2016/06117-5 ; Processo FAPESP: 2016/11470-6 ; CNPq: 304853/2018-6 ; Photofunctionalization mediated by ultraviolet (UV) light seems to be a promising approach to improve the physico-chemical characteristics and the biological response of titanium (Ti) dental implants. Seeing that photofunctionalization is able to remove carbon from the surface, besides to promote reactions on the titanium dioxide (TiO2) layer, coating the Ti with a stable TiO2 film could potentialize the UV effect. Thus, here we determined the impact of UV-photofunctionalized mixed-phase (anatase and rutile) TiO2 films on the physico-chemical properties of Ti substrate and cell biology. Mixed-phase TiO2 films were grown by radiofrequency magnetron sputtering on commercially pure titanium (cpTi) discs, and samples were divided as follow: cpTi (negative control), TiO2 (positive control), cpTi UV, TiO2 UV (experimental). Photofunctionalization was performed using UVA (360 nm - 40 W) and UVC (250 nm - 40 W) lamps for 48 h. Surfaces were analyzed in terms of morphology, topography, chemical composition, crystalline phase, wettability and surface free energy. Pre-osteoblastic cells (MC3T3E1) were used to assess cell morphology and adhesion, metabolism, mineralization potential and cytokine secretion (IFN-γ, TNF-α, IL-4, IL-6 and IL-17). TiO2-coated surfaces exhibited granular surface morphology and greater roughness. Photofunctionalization increased wettability (p < 0.05) and surface free energy (p < 0.001) on both surface conditions. TiO2-treated groups featured normal cell morphology and spreading, and greater cellular metabolic activity at 2 and 4 days (p < 0.05), whereas UV-photofunctionalized surfaces enhanced cell metabolism, cell adhered area, and calcium deposition (day 14) (p < 0.05). In general, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1873-0191 0928-4931 |
| Relation: | Materials Science and Engineering C; http://dx.doi.org/10.1016/j.msec.2020.111438; Materials Science and Engineering C, v. 118.; http://hdl.handle.net/11449/205161; 2-s2.0-85090138541 |
| DOI: | 10.1016/j.msec.2020.111438 |
| الاتاحة: | http://hdl.handle.net/11449/205161 https://doi.org/10.1016/j.msec.2020.111438 |
| رقم الانضمام: | edsbas.58C66FEE |
| قاعدة البيانات: | BASE |
| تدمد: | 18730191 09284931 |
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| DOI: | 10.1016/j.msec.2020.111438 |