Academic Journal
References
| العنوان: | References |
|---|---|
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.clinchem.org/content/50/4/775.full.pdf. |
| المجموعة: | CiteSeerX |
| مصطلحات موضوعية: | Rapid cycle real-time PCR methods and applications (genetics and oncol |
| الوصف: | produce a peak within 1–2 °C of the predicted Tm. This allows Tm peaks, which indicate the presence or absence of a PCR product, to be strategically placed within the typical melting curve range of 45–70 °C for hybridization probes. Over this melting curve range we feel it should be possible to clearly distinguish three peaks in a single channel. This potentially makes it possible to multiplex six mutations in two capillaries when both fluorescent channels are used. This enhances the capabilities of the LightCycler for mutation detection; similar strategies could also be used for other real-time systems. The results presented here demonstrate the ability to carry out multiplex mutation detection by use of a com-bination of ARMS PCR and real-time detection. A labora-tory currently using ARMS PCR in a diagnostic setting can quite easily convert the standard ARMS PCR to a real-time ARMS PCR. This could have a major advantage in time savings and reduce the handling of potential carcinogenic ethidium bromide. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.601.2164; http://www.clinchem.org/content/50/4/775.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.601.2164 http://www.clinchem.org/content/50/4/775.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.588BF3E7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |