Dissertation/ Thesis
Phylogenetic and biochemical insights into the diversity of TET peptidases in Archaea
| العنوان: | Phylogenetic and biochemical insights into the diversity of TET peptidases in Archaea |
|---|---|
| المؤلفون: | Chagny, Emilie |
| بيانات النشر: | Université de Bretagne Occidentale |
| سنة النشر: | 2024 |
| المجموعة: | Ifremer (French Research Institute for Exploitation of the Sea): Archimer |
| مصطلحات موضوعية: | Peptidases TET, Archées, protéolyse, analyse de séquence, enzymologie, TET peptidases, Archaea, proteolysis, sequence analysis, enzymology |
| الوصف: | TET peptidases are giant aminopeptidases ubiquitous accross all domains life, proposed to be involved in intracellular proteolysis, acting downstream of the proteasome. Structural studies have shown that these enzymes form hollow dodecameric tetrahedral complexes. In Archaea, TET peptidases have primarily been studied in Thermococcales species, revealing four distinct types of TETs. However, the prevalence and functional diversity of these peptidases in other archaeal lineages remain poorly understood, and their precise biological role is still unclear. In this work, structure-based identification criteria are introduced, enabling highthroughput screening for TETs. By applying these criteria to 4,016 archaeal genomes, the first comprehensive analysis of TET distribution in Archaea was conducted, uncovering an unsuspected diversity. Through phylogenetic analysis, a classification of archaeal TETs in eleven families is proposed. Biochemical characterization of six new enzymes from previously undescribed families and covering a wide taxonomic range of archaeal species offers an extensive overview of the functional diversity of TETs. Finally, by combining biochemical and phylogenetic data, the evolutionary history of these peptidases is addressed, offering new insights into their potential biological roles. ; Les peptidases TET sont des aminopeptidases géantes présentes dans les trois domaines du vivant. Elles sont supposées intervenir dans la protéolyse intracellulaire, en aval du protéasome. Des études structurales ont montré que ces enzymes forment des complexes tétraédriques dodécamériques creux. Chez les Archées, les peptidases TET ont principalement été étudiées chez des espèces de l’ordre des Thermococcales, révélant quatre types distincts de TET. Cependant, la répartition et la diversité fonctionnelle de ces peptidases dans les autres lignées archéennes restent mal connue, et leur rôle biologique précis est toujours indéterminé. Dans cette étude, des critères d’identification basés sur des éléments ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://archimer.ifremer.fr/doc/00931/104274/116339.pdf; https://archimer.ifremer.fr/doc/00931/104274/ |
| الاتاحة: | https://archimer.ifremer.fr/doc/00931/104274/116339.pdf https://archimer.ifremer.fr/doc/00931/104274/ |
| Rights: | info:eu-repo/semantics/openAccess ; restricted use |
| رقم الانضمام: | edsbas.5886B287 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |