Academic Journal
Crystallization of 5-aminolaevulinic acid dehydratase from Escherichia coli and Saccharomyces cerevisiae and preliminary X-ray characterization of the crystals
| العنوان: | Crystallization of 5-aminolaevulinic acid dehydratase from Escherichia coli and Saccharomyces cerevisiae and preliminary X-ray characterization of the crystals |
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| المؤلفون: | Erskine, Peter T., Senior, Natalie, Maignan, S., Cooper, J., Lambert, Richard, Lewis, Gareth, Spencer, Paul, Awan, Sarah, Warren, Martin J., Tickle, Ian J., Thomas, P., Wood, S.P., Shoolingin-Jordan, Peter M. |
| بيانات النشر: | Cambridge Univ Press, 40 West 20th Street, New York, NY 10011-4211 |
| سنة النشر: | 1997 |
| المجموعة: | University of Kent: KAR - Kent Academic Repository |
| مصطلحات موضوعية: | Q Science |
| الوصف: | 5-Aminolaevulinic acid dehydratase (ALAD) catalyzes the formation of porphobilinogen from two molecules of 5-aminolaevulinic acid. Both Escherichia coli and Saccharomyces cerevisiae ALADs are homo-octameric enzymes which depend on Zn2+ for catalytic activity and are potently inhibited by lead ions. The E. coli enzyme crystallized in space group I422 (unit cell dimensions a = b = 130.7 A, c = 142.4 A). The best crystals were obtained in the presence of the covalently bound inhibitor laevulinic acid. The yeast enzyme (expressed in E. coli) crystallized in the same space group (I422) but with a smaller unit cell volume (a = b = 103.7 A, c = 167.7 A). High resolution synchrotron data sets were obtained from both E. coli and yeast ALAD crystals by cryocooling to 100 K. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | Erskine, Peter T., Senior, Natalie, Maignan, S., Cooper, J., Lambert, Richard, Lewis, Gareth, Spencer, Paul, Awan, Sarah, Warren, Martin J., Tickle, Ian J., and others. Thomas, P., Wood, S.P., and Shoolingin-Jordan, Peter M. (hide) (1997) Crystallization of 5-aminolaevulinic acid dehydratase from Escherichia coli and Saccharomyces cerevisiae and preliminary X-ray characterization of the crystals. Protein Science, 6 (8). pp. 1774-6. ISSN 0961-8368. (doi:10.1002/pro.5560060820 ) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:11092 ) |
| DOI: | 10.1002/pro.5560060820 |
| الاتاحة: | https://kar.kent.ac.uk/11092/ https://doi.org/10.1002/pro.5560060820 |
| رقم الانضمام: | edsbas.5876AF4E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/pro.5560060820 |
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