Chemoproteomic discovery of a human RNA ligase

التفاصيل البيبلوغرافية
العنوان:	Chemoproteomic discovery of a human RNA ligase
المؤلفون:	Yuan, Yizhi, Stumpf, Florian M., Schmidt, Olivia, Saumer, Philip, Huber, Luisa B., Frese, Matthias, Höllmüller, Eva, Scheffner, Martin, Stengel, Florian, Diederichs, Kay, Marx, Andreas
المصدر:	Nature Communications. Nature Publishing Group. 2023, 14, 842. eISSN 2041-1723. Available under: doi:10.1038/s41467-023-36451-x
سنة النشر:	2023
المجموعة:	University of Konstanz: Konstanz Online Publication Server (KOPS)
مصطلحات موضوعية:	ddc:570
الوصف:	RNA ligases are present across all forms of life. While enzymatic RNA ligation between 5′-PO4 and 3′-OH termini is prevalent in viruses, fungi, and plants, such RNA ligases are yet to be identified in vertebrates. Here, using a nucleotide-based chemical probe targeting human AMPylated proteome, we have enriched and identified the hitherto uncharacterised human protein chromosome 12 open reading frame 29 (C12orf29) as a human enzyme promoting RNA ligation between 5′-PO4 and 3′-OH termini. C12orf29 catalyses ATP-dependent RNA ligation via a three-step mechanism, involving tandem auto- and RNA AMPylation. Knock-out of C12ORF29 gene impedes the cellular resilience to oxidative stress featuring concurrent RNA degradation, which suggests a role of C12orf29 in maintaining RNA integrity. These data provide the groundwork for establishing a human RNA repair pathway. ; published
نوع الوثيقة:	article in journal/newspaper
وصف الملف:	application/pdf
اللغة:	English
ردمك:	978-1-83706-174-7 1-83706-174-2
Relation:	http://nbn-resolving.de/urn:nbn:de:bsz:352-2-9hn573vvaei94; http://dx.doi.org/10.1038/s41467-023-36451-x
DOI:	10.1038/s41467-023-36451-x
الاتاحة:	http://nbn-resolving.de/urn:nbn:de:bsz:352-2-9hn573vvaei94 https://doi.org/10.1038/s41467-023-36451-x
Rights:	https://rightsstatements.org/page/InC/1.0/
رقم الانضمام:	edsbas.586CAAD
قاعدة البيانات:	BASE

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الوصف
ردمك:	9781837061747 1837061742
DOI:	10.1038/s41467-023-36451-x