Academic Journal
Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation
| العنوان: | Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation |
|---|---|
| المؤلفون: | Yao, Changfu, Wang, Chao, Li, Yeran, Zavortink, Michael, Archambault, Vincent, Girton, Jack, Johansen, Kristen M., Johansen, Jorgen |
| المصدر: | Biochemistry, Biophysics and Molecular Biology Publications |
| بيانات النشر: | Iowa State University Digital Repository |
| سنة النشر: | 2018 |
| المجموعة: | Digital Repository @ Iowa State University |
| مصطلحات موضوعية: | Biochemistry, Biophysics, Molecular Biology, Structural Biology |
| الوصف: | In Drosophila it has recently been demonstrated that a spindle matrix in the form of a membrane-less macromolecular assembly embeds the microtubule-based spindle apparatus. In addition, two of its constituents, Megator and Chromator, were shown to function as spatial regulators of spindle checkpoint proteins. However, whether the spindle matrix plays a wider functional role in spatially regulating cell cycle progression factors was unknown. Here using a live imaging approach we provide evidence that a number of key cell cycle proteins such as Cyclin B, Polo, and Ran co-localize with the spindle matrix during mitosis. Furthermore, prevention of spindle matrix formation by injection of a function blocking antibody against the spindle matrix protein Chromator results in cell cycle arrest prior to nuclear envelope breakdown. In such embryos the spatial dynamics of Polo and Cyclin B enrichment at the nuclear rim and kinetochores is abrogated and Polo is not imported into the nucleus. This is in contrast to colchicine-arrested embryos where the wild-type dynamics of these proteins are maintained. Taken together these results suggest that spindle matrix formation may be a general requirement for the localization and proper dynamics of cell cycle factors promoting signaling events leading to cell cycle progression. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://lib.dr.iastate.edu/bbmb_ag_pubs/233; https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1240&context=bbmb_ag_pubs |
| الاتاحة: | https://lib.dr.iastate.edu/bbmb_ag_pubs/233 https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1240&context=bbmb_ag_pubs |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.58667F13 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |