Book
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
| العنوان: | Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
|---|---|
| المؤلفون: | Sara Pereira, Rita S. Santos, Luís Moreira, Nuno M. Guimarães, Kevin Braeckmans, Stefaan C. De Smedt, Nuno F. Azevedo |
| المساهمون: | Faculdade de Engenharia |
| سنة النشر: | 2021 |
| المجموعة: | Repositório Aberto da Universidade do Porto |
| الوصف: | Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature. |
| نوع الوثيقة: | book |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Fluorescence In-Situ Hybridization (FISH) for Microbial Cells. Methods in Molecular Biology; https://hdl.handle.net/10216/140770; P-00T-MEK |
| DOI: | 10.1007/978-1-0716-1115-9_6 |
| الاتاحة: | https://hdl.handle.net/10216/140770 https://doi.org/10.1007/978-1-0716-1115-9_6 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.5853760D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-0716-1115-9_6 |
|---|