Academic Journal
Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of H2O2 enhances their ability to treat intervertebral disc degeneration
| العنوان: | Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of H2O2 enhances their ability to treat intervertebral disc degeneration |
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| المؤلفون: | Zhang, Yu-yao, Hu, Zhi-lei, Qi, Yu-han, Li, Hai-yin, Chang, Xian, Gao, Xiao-xin, Liu, Chen-hao, Li, Yue-yang, Lou, Jin-hui, Zhai, Yu, Li, Chang-qing |
| المساهمون: | National Natural Science Foundation of China |
| المصدر: | Stem Cell Research & Therapy ; volume 13, issue 1 ; ISSN 1757-6512 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| الوصف: | Background Nucleus pulposus mesenchymal stem cells (NPMSCs) transplantation is a promising treatment for intervertebral disc degeneration (IVDD). However, the transplanted NPMSCs exhibited weak cell proliferation, high cell apoptosis, and a low ability to resist the harsh microenvironment of the degenerated intervertebral disc. There is an urgent need to explore feasible methods to enhance the therapeutic efficacy of NPMSCs transplantation. Objective To identify the optimal concentration for NPMSCs pretreatment with hydrogen peroxide (H 2 O 2 ) and explore the therapeutic efficacy of NPMSCs transplantation using H 2 O 2 pretreatment in IVDD. Methods Rat NPMSCs were pretreated with different concentrations (range from 25 to 300 μM) of H 2 O 2 . The proliferation, reactive oxygen species (ROS) level, and apoptosis of NPMSCs were detected by cell counting kit-8 (CCK-8) assay, 5-ethynyl-2′-deoxyuridine (EdU) staining, and flow cytometry in vitro. The underlying signalling pathways were explored utilizing Western blotting. A rat needle puncture-stimulated IVDD model was established. X-ray, histological staining, and a multimode small animal live imaging system were used to evaluate the therapeutic effect of H 2 O 2 -pretreated NPMSCs in vivo. Results NPMSCs pretreated with 75 μM H 2 O 2 demonstrated the strongest elevated cell proliferation by inhibiting the Hippo pathway ( P < 0.01). Meanwhile, 75 μM H 2 O 2 -pretreated NPMSCs exhibited significantly enhanced antioxidative stress ability ( P < 0.01), which is related to downregulated Brd4 and Keap1 and upregulated Nrf2. NPMSCs pretreated with 75 μM H 2 O 2 also exhibited distinctly decreased apoptosis ( P < 0.01). In vivo experiments verified that 75 μM H 2 O 2 -pretreated NPMSCs-transplanted rats exhibited an enhanced disc height index (DHI% = 90.00 ± 4.55, P < 0.01) and better histological morphology (histological score = 13.5 ± 0.5, P < 0.01), which means 75 μM H 2 O 2 -pretreated NPMSCs can better adapt to the environment of degenerative ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s13287-022-03031-7 |
| DOI: | 10.1186/s13287-022-03031-7.pdf |
| DOI: | 10.1186/s13287-022-03031-7/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s13287-022-03031-7 https://link.springer.com/content/pdf/10.1186/s13287-022-03031-7.pdf https://link.springer.com/article/10.1186/s13287-022-03031-7/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.5823B8E9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13287-022-03031-7 |
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