Academic Journal
Extracellular Vesicles From Human Urine-Derived Stem Cells Ameliorate Erectile Dysfunction in a Diabetic Rat Model by Delivering Proangiogenic MicroRNA
| العنوان: | Extracellular Vesicles From Human Urine-Derived Stem Cells Ameliorate Erectile Dysfunction in a Diabetic Rat Model by Delivering Proangiogenic MicroRNA |
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| المؤلفون: | Ouyang, Bin, Xie, Yun, Zhang, Chi, Deng, Cuncan, Lv, Linyan, Yao, Jiahui, Zhang, Yuanyuan, Liu, Guihua, Deng, Junhong, Deng, Chunhua |
| المساهمون: | Natural Science Foundation of Guangdong Province, National Natural Science Foundation of China |
| المصدر: | Sexual Medicine ; volume 7, issue 2, page 241-250 ; ISSN 2050-1161 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2019 |
| الوصف: | Introduction Stem cell therapies represent a promising new frontier for the treatment of refractory diabetic erectile dysfunction (DED). The use of stem cell-derived extracellular vesicles (EVs) is a novel strategy for cell-free stem cell therapy. We have reported that urine-derived stem cells (USCs) can improve DED; however, the therapeutic effects of EVs secreted by USCs (USC-EVs) remain unknown. Aim To determine the therapeutic effects of USC-EVs on DED in a rat model. Methods USC-EVs were isolated from conditioned medium by ultracentrifugation. DED was induced in male Sprague–Dawley rats via an intraperitoneal injection of streptozotocin. Sixteen DED rats were divided into phosphate-buffered saline (PBS) and USC-EV groups. Eight normal rats served as the normal control group. PBS or USC-EVs were transplanted into the corpora cavernosa in the corresponding groups. Main Outcome Measure Intracavernosal pressure (ICP), mean arterial pressure (MAP), expression of endothelial markers (CD31), endothelial nitric oxide synthase (eNOS), phospho-eNOS, and neural nitric oxide synthase (nNOS) were assessed in each group. Masson’s trichrome staining was used to determine the collagen deposition and ratio of smooth muscle cells to collagen. The microRNA (miRNA) cargo of USC-EVs was characterized by high-throughput RNA sequencing. Results Recovery of erectile function was observed in the USC-EV group, as represented by improved ICP and ICP/MAP ratio. CD31, eNOS, phospho-eNOS, and nNOS expression in the penis was significantly improved in the USC-EV group. In addition, the ratio of smooth muscle to collagen was significantly increased in the USC-EV group. RNA sequencing revealed that USC-EVs were enriched for distinct classes of miRNA (miR-21-5p, let-7 family, miR-10 family, miR-30 family, and miR-148a-3p) that promote angiogenesis. Conclusion USC-EV transplantation can ameliorate DED in rats. Its mechanism may involve the delivery of proangiogenic miRNA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.esxm.2019.02.001 |
| الاتاحة: | https://doi.org/10.1016/j.esxm.2019.02.001 https://api.elsevier.com/content/article/PII:S2050116119300273?httpAccept=text/xml https://api.elsevier.com/content/article/PII:S2050116119300273?httpAccept=text/plain https://academic.oup.com/smoa/article-pdf/7/2/241/48791893/smoa_7_2_241.pdf |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ ; https://www.elsevier.com/tdm/userlicense/1.0/ ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.57F17EF8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.esxm.2019.02.001 |
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