التفاصيل البيبلوغرافية
| العنوان: |
Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells |
| المؤلفون: |
Alexey S. Nagel, Natalia V. Rudenko, Polina N. Luchkina, Anna P. Karatovskaya, Anna V. Zamyatina, Zhanna I. Andreeva-Kovalevskaya, Alexander V. Siunov, Fedor A. Brovko, Alexander S. Solonin |
| المصدر: |
Molecules; Volume 28; Issue 8; Pages: 3581 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
pore-forming toxin, monoclonal antibody, agglutination, three-dimensional protein structure modeling |
| جغرافية الموضوع: |
agris |
| الوصف: |
Hemolysin II (HlyII) is one of the virulence factors of the opportunistic bacterium Bacillus cereus belonging to the group of β-pore-forming toxins. This work created a genetic construct encoding a large C-terminal fragment of the toxin (HlyIILCTD, M225–I412 according to the numbering of amino acid residues in HlyII). A soluble form of HlyIILCTD was obtained using the SlyD chaperone protein. HlyIILCTD was first shown to be capable of agglutinating rabbit erythrocytes. Monoclonal antibodies against HlyIILCTD were obtained by hybridoma technology. We also proposed a mode of rabbit erythrocyte agglutination by HlyIILCTD and selected three anti-HlyIILCTD monoclonal antibodies that inhibited the agglutination. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Chemical Biology; https://dx.doi.org/10.3390/molecules28083581 |
| DOI: |
10.3390/molecules28083581 |
| الاتاحة: |
https://doi.org/10.3390/molecules28083581 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.57CB85B5 |
| قاعدة البيانات: |
BASE |