Academic Journal
(p)ppGpp Regulates a Bacterial Nucleosidase by an Allosteric Two-Domain Switch
| العنوان: | (p)ppGpp Regulates a Bacterial Nucleosidase by an Allosteric Two-Domain Switch |
|---|---|
| المؤلفون: | Zhang, Yong Everett, Bærentsen, René Lysdal, Fuhrer, Tobias, Sauer, Uwe, Gerdes, Kenn, Brodersen, Ditlev Egeskov |
| المصدر: | Molecular cell 74(6), 1239 - 1249.e4 (2019). doi:10.1016/j.molcel.2019.03.035 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | DESY Publication Database (PUBDB) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/610 |
| جغرافية الموضوع: | DE |
| الوصف: | The stringent response alarmones pppGpp and ppGpp are essential for rapid adaption of bacterial physiology to changes in the environment. In Escherichia coli, the nucleosidase PpnN (YgdH) regulates purine homeostasis by cleaving nucleoside monophosphates and specifically binds (p)ppGpp. Here, we show that (p)ppGpp stimulates the catalytic activity of PpnN both in vitro and in vivo causing accumulation of several types of nucleobases during stress. The structure of PpnN reveals a tetramer with allosteric (p)ppGpp binding sites located between subunits. pppGpp binding triggers a large conformational change that shifts the two terminal domains to expose the active site, providing a structural rationale for the stimulatory effect. We find that PpnN increases fitness and adjusts cellular tolerance to antibiotics and propose a model in which nucleotide levels can rapidly be adjusted during stress by simultaneous inhibition of biosynthesis and stimulation of degradation, thus achieving a balanced physiological response to constantly changing environments. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/pmid:31023582; info:eu-repo/semantics/altIdentifier/issn/1097-4164; info:eu-repo/semantics/altIdentifier/wos/WOS:000472231600014; info:eu-repo/semantics/altIdentifier/issn/1097-2765; https://bib-pubdb1.desy.de/record/425966; https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2019-03509%22 |
| الاتاحة: | https://bib-pubdb1.desy.de/record/425966 https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2019-03509%22 |
| Rights: | info:eu-repo/semantics/embargoedAccess |
| رقم الانضمام: | edsbas.579FEB87 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |