التفاصيل البيبلوغرافية
| العنوان: |
Comparison of the IP-MS assay for VP40 peptide variants with an RT-PCR assay for the GP gene. |
| المؤلفون: |
Qingbo Shu (1555585), Tara Kenny (584879), Jia Fan (178422), Christopher J. Lyon (7339508), Lisa H. Cazares (2352271), Tony Y. Hu (2625703) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Physiology, Biotechnology, Ecology, Immunology, Infectious Diseases, Plant Biology, Virology, Chemical Sciences not elsewhere classified, produced affinity variations, human plasma spiked, disease severity caused, coupled mass spectrometry, available bioinformatic tools, antigenicity prediction based, 5 × 10, tafv ), non, taï forest virus, plausible bombali virus, tof ms instrument, viral burden quantification, six ebolavirus species, div >< p, 625 – 6, 5 , recombinant vp40 protein, high specificity assay, ms assay limit, vp40 antigen detection |
| الوصف: |
Both assays employ four steps for sample analysis. ( A ) In the IP-MS assay for VP40 peptide variants, samples are inactivated using a method described by 2005 CDC Interim Guidance and digested to release the target VP40 peptides, which are then immunoprecipitated, and analyzed by mass spectrometry to detect mass differences among the peptide 8 and 12 sequences encoded by different ebolavirus species. The VP40 p8 and p12 target peptides and internal standard (IS) peptide are captured during immunoprecipitation (IP) by target-specific antibody-coupled magnetic beads. The peak area ratio between known amounts of VP40 p8 (blue peak) and its corresponding IS peptide (purple peak) is used to generate a standard curve to permit quantification of endogenous VP40 in clinical samples. ( B ) In the RT-PCR assay for the GP gene, a carrier RNA and a high concentration internal control are spiked into the sample before extraction. Each extracted RNA sample is tested with the internal control RT-PCR RNA Assay to evaluate the yield of the spiked-in internal control. If the internal control amplified within manufacturer-designated ranges, further quantitative analysis of the viral target is performed using standard procedures. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Comparison_of_the_IP-MS_assay_for_VP40_peptide_variants_with_an_RT-PCR_assay_for_the_GP_gene_/16957602 |
| DOI: |
10.1371/journal.ppat.1010039.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1010039.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.577B4CE3 |
| قاعدة البيانات: |
BASE |