Academic Journal
Exploring nuclear pore complex molecular architecture by immuno-electron microscopy using Xenopus oocytes
| العنوان: | Exploring nuclear pore complex molecular architecture by immuno-electron microscopy using Xenopus oocytes |
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| المؤلفون: | Panté, Nelly, Fahrenkrog, Birthe |
| المصدر: | Methods in cell biology, 122 |
| سنة النشر: | 2014 |
| المجموعة: | DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: | Biologie cellulaire, Active Transport, Cell Nucleus, Animals, Antibodies, Monoclonal -- immunology, Epitopes -- immunology, Female, Gold Colloid, Microinjections -- methods, Microscopy, Immunoelectron -- methods, Microtomy, Nuclear Pore -- chemistry, Nuclear Pore Complex Proteins -- immunology, Oocytes -- cytology, Protein Structure, Tertiary, Staining and Labeling, Xenopus laevis, Electron microscopy, Immunolabeling, Nuclear pore complex, Nucleocytoplasmic transport, Xenopus oocytes |
| الوصف: | Xenopus oocytes are large in size and perfectly suited for microinjection experiments. Their nuclei, which can be readily isolated manually, are characterized by an extremely high density of nuclear pore complexes (NPCs). Therefore, Xenopus oocytes are an excellent system to study NPC structure and molecular architecture, as well as nucleocytoplasmic transport on an ultrastructural level. A wide range of electron microscopy (EM) techniques can be employed to do so and thin-sectioning immuno-EM has been proven to be a powerful tool in this context. NPCs are composed of multiple copies of a set of about 30 different nucleoporins, which are often large, multidomain proteins. Their complex organization within NPCs can be unraveled by using domain-specific antibodies to individual nucleoporins in combination with microinjection and expression of epitope-tagged nucleoporins. Here, we describe the immuno-EM methods using Xenopus oocyte that allow for precise ultrastructural localization of nucleoporins within the structure of the NPC. © 2014 Elsevier Inc. ; SCOPUS: ar.k ; info:eu-repo/semantics/published |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1 full-text file(s): application/pdf |
| اللغة: | English |
| ردمك: | 978-0-12-417160-2 0-12-417160-5 |
| Relation: | uri/info:doi/10.1016/B978-0-12-417160-2.00004-7; uri/info:pii/B9780124171602.000047; uri/info:pmid/24857726; uri/info:scp/84900854276; https://dipot.ulb.ac.be/dspace/bitstream/2013/183282/4/Elsevier_166909.pdf; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/183282 |
| الاتاحة: | http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/183282 https://dipot.ulb.ac.be/dspace/bitstream/2013/183282/4/Elsevier_166909.pdf |
| رقم الانضمام: | edsbas.576302FA |
| قاعدة البيانات: | BASE |
| ردمك: | 9780124171602 0124171605 |
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