التفاصيل البيبلوغرافية
| العنوان: |
Longitudinal two-photon imaging in somatosensory cortex of behaving mice reveals dendritic spine formation enhancement by subchronic administration of low-dose ketamine |
| المؤلفون: |
Pryazhnikov, Evgeny, Mugantseva, Ekaterina, Casarotto, Plinio, Kolikova, Julia, Fred, Senem Merve, Toptunov, Dmytro, Afzalov, Ramil, Hotulainen, Pirta, Voikar, Vootele, Terry-Lorenzo, Ryan, Engel, Sharon, Kirov, Sergei, Castren, Eero, Khiroug, Leonard |
| المساهمون: |
Neuroscience Center, Helsinki In Vivo Animal Imaging Platform (HAIP), Eero Castren / Principal Investigator |
| بيانات النشر: |
Nature Publishing Group |
| سنة النشر: |
2019 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
RESISTANT MAJOR DEPRESSION, SYNAPSE FORMATION, IN-VIVO, RECEPTORS, MATURATION, PHENCYCLIDINE, METABOLITES, PLASTICITY, BRAIN, Neurosciences |
| الوصف: |
Ketamine, a well-known anesthetic, has recently attracted renewed attention as a fast-acting antidepressant. A single dose of ketamine induces rapid synaptogenesis, which may underlie its antidepressant effect. To test whether repeated exposure to ketamine triggers sustained synaptogenesis, we administered a sub-anesthetic dose of ketamine (10 mg/kg i.p.) once-daily for 5 days, and repeatedly imaged dendritic spines of the YFP-expressing pyramidal neurons in somatosensory cortex of awake female mice using in vivo two-photon microscopy. We found that the spine formation rate became significantly higher at 72-132 h after the first ketamine injection (but not at 6-24 h), while the rate of elimination of pre-existing spines remained unchanged. In contrast to the net gain of spines observed in ketamine-treated mice, the vehicle-injected control mice exhibited a net loss typical for young-adult animals undergoing synapse pruning. Ketamine-induced spinogenesis was correlated with increased PSD-95 and phosphorylated actin, consistent with formation of new synapses. Moreover, structural synaptic plasticity caused by ketamine was paralleled by a significant improvement in the nest building behavioral assay. Taken together, our data show that subchronic low-dose ketamine induces a sustained shift towards spine formation. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
The study was sponsored by Sunovion Pharmaceuticals Inc. Funding for PC, SMF and EC was provided by ERC grant No 322742 - iPLASTICITY, Sigrid Juselius foundation and Academy of Finland grants #294710 and #307416. Funding for SAK was provided by the NIH grant NS083858. The authors thank Prof. Anton Bespalov and Prof. Mark Reimers for their valuable advices and comments.; Pryazhnikov , E , Mugantseva , E , Casarotto , P , Kolikova , J , Fred , S M , Toptunov , D , Afzalov , R , Hotulainen , P , Voikar , V , Terry-Lorenzo , R , Engel , S , Kirov , S , Castren , E & Khiroug , L 2018 , ' Longitudinal two-photon imaging in somatosensory cortex of behaving mice reveals dendritic spine formation enhancement by subchronic administration of low-dose ketamine ' , Scientific Reports , vol. 8 , 6464 . https://doi.org/10.1038/s41598-018-24933-8; ORCID: /0000-0002-1402-2791/work/54147280; ORCID: /0000-0002-1090-4631/work/54150978; ORCID: /0000-0003-4201-8666/work/54151371; ORCID: /0000-0003-1992-5014/work/54151765; http://hdl.handle.net/10138/299100; 5e655f72-d57d-4b56-9a46-6fc1d2a78484; 85045958529; 000430662200009 |
| الاتاحة: |
http://hdl.handle.net/10138/299100 |
| Rights: |
cc_by ; info:eu-repo/semantics/openAccess ; openAccess |
| رقم الانضمام: |
edsbas.56FA4B6A |
| قاعدة البيانات: |
BASE |