Academic Journal
Diet-Stimulated Marrow Adiposity Fails to Worsen Early, Age-Related Bone Loss
| العنوان: | Diet-Stimulated Marrow Adiposity Fails to Worsen Early, Age-Related Bone Loss |
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| المؤلفون: | McGrath, Cody, Little-Letsinger, Sarah E., Pagnotti, Gabriel M., Sen, Buer, Xie, Zhihui, Uzer, Gunes, Uzer, Guniz B., Zong, Xiaopeng, Styner, Martin A., Rubin, Janet, Styner, Maya |
| المصدر: | Obesity Facts ; volume 17, issue 2, page 145-157 ; ISSN 1662-4025 1662-4033 |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 2024 |
| الوصف: | Introduction: Longitudinal effect of diet-induced obesity on bone is uncertain. Prior work showed both no effect and a decrement in bone density or quality when obesity begins prior to skeletal maturity. We aimed to quantify long-term effects of obesity on bone and bone marrow adipose tissue (BMAT) in adulthood. Methods: Skeletally mature, female C57BL/6 mice (n = 70) aged 12 weeks were randomly allocated to low-fat diet (LFD; 10% kcal fat; n = 30) or high-fat diet (HFD; 60% kcal fat; n = 30), with analyses at 12, 15, 18, and 24 weeks (n = 10/group). Tibial microarchitecture was analyzed by µCT, and volumetric BMAT was quantified via 9.4T MRI/advanced image analysis. Histomorphometry of adipocytes and osteoclasts, and qPCR were performed. Results: Body weight and visceral white adipose tissue accumulated in response to HFD started in adulthood. Trabecular bone parameters declined with advancing experimental age. BV/TV declined 22% in LFD (p = 0.0001) and 17% in HFD (p = 0.0022) by 24 weeks. HFD failed to appreciably alter BV/TV and had negligible impact on other microarchitecture parameters. Both dietary intervention and age accounted for variance in BMAT, with regional differences: distal femoral BMAT was more responsive to diet, while proximal femoral BMAT was more attenuated by age. BMAT increased 60% in the distal metaphysis in HFD at 18 and 24 weeks (p = 0.0011). BMAT in the proximal femoral diaphysis, unchanged by diet, decreased 45% due to age (p = 0.0002). Marrow adipocyte size via histomorphometry supported MRI quantification. Osteoclast number did not differ between groups. Tibial qPCR showed attenuation of some adipose, metabolism, and bone genes. A regulator of fatty acid β-oxidation, cytochrome C (CYCS), was 500% more abundant in HFD bone (p < 0.0001; diet effect). CYCS also increased due to age, but to a lesser extent. HFD mildly increased OCN, TRAP, and SOST. Conclusions: Long-term high fat feeding after skeletal maturity, despite upregulation of visceral adiposity, body weight, and BMAT, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1159/000536159 |
| DOI: | 10.1159/000536159/4188077/000536159.pdf |
| الاتاحة: | http://dx.doi.org/10.1159/000536159 https://karger.com/ofa/article-pdf/doi/10.1159/000536159/4188077/000536159.pdf |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ ; https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.5660EB04 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000536159 |
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