التفاصيل البيبلوغرافية
| العنوان: |
Identification of a Serine Proteinase Homolog (Sp-SPH) Involved in Immune Defense in the Mud Crab Scylla paramamosain |
| المؤلفون: |
Zhang, Qiu-xia, Liu, Hai-peng, Chen, Rong-yuan, Shen, Kai-li, Wang, Ke-jian, 刘海鹏, 陈荣元, 王克坚 |
| المصدر: |
http://dx.doi.org/10.1371/journal.pone.0063787. |
| بيانات النشر: |
PUBLIC LIBRARY SCIENCE |
| سنة النشر: |
2013 |
| المجموعة: |
Xiamen University Institutional Repository |
| مصطلحات موضوعية: |
CRAYFISH PACIFASTACUS-LENIUSCULUS, RECOGNITION PROTEIN, CELL-ADHESION, MANDUCA-SEXTA, EPITHELIAL-CELLS, ACTIVATION, DROSOPHILA, PHENOLOXIDASE, MASQUERADE, SHRIMP |
| الوصف: |
National Natural Science Foundation of China [41006077]; Research Fund for the Doctoral Program of Higher Education [20100121120028]; Science Foundation of the Fujian Province, China [2010NZ0002-3, JA10002]; Public Science and Technology Research Funds Projects of Ocean [201105027] ; Clip domain serine proteinase homologs are involved in many biological processes including immune response. To identify the immune function of a serine proteinase homolog (Sp-SPH), originally isolated from hemocytes of the mud crab, Scylla paramamosain, the Sp-SPH was expressed recombinantly and purified for further studies. It was found that the Sp-SPH protein could bind to a number of bacteria (including Aeromonas hydrophila, Escherichia coli, Staphylococcus aureus, Vibrio fluvialis, Vibrio harveyi and Vibrio parahemolyticus), bacterial cell wall components such as lipopolysaccharide or peptidoglycan (PGN), and beta-1, 3-glucan of fungus. But no direct antibacterial activity of Sp-SPH protein was shown by using minimum inhibitory concentration or minimum bactericidal concentration assays. Nevertheless, the Sp-SPH protein was found to significantly enhance the crab hemocyte adhesion activity (paired t-test, P<0.05), and increase phenoloxidase activity if triggered by PGN in vitro (paired t-test, P<0.05). Importantly, the Sp-SPH protein was demonstrated to promote the survival rate of the animals after challenge with A. hydrophila or V. parahemolyticus which were both recognized by Sp-SPH protein, if pre-incubated with Sp-SPH protein, respectively. Whereas, the crabs died much faster when challenged with Vibrio alginolyiicus, a pathogenic bacterium not recognized by Sp-SPH protein, compared to those of crabs challenged with A. hydrophila or V. parahemolyticus when pre-coated with Sp-SPH protein. Taken together, these data suggested that Sp-SPH molecule might play an important role in immune defense against bacterial infection in the mud crab S. paramamosain. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
PLOS ONE, 2013,8(5); WOS:000319733000024; http://dspace.xmu.edu.cn/handle/2288/88028 |
| الاتاحة: |
http://dspace.xmu.edu.cn/handle/2288/88028 |
| رقم الانضمام: |
edsbas.5614CC4 |
| قاعدة البيانات: |
BASE |