Academic Journal
Dual actions of brain-derived neurotrophic factor on GABAergic transmission in cerebellar Purkinje neurons
| العنوان: | Dual actions of brain-derived neurotrophic factor on GABAergic transmission in cerebellar Purkinje neurons |
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| المؤلفون: | Wang, JJ, Morozov, A, Ke, Y, Ip, NY, Yung, WH, Huang, Y, Ko, H, Cheung, ZH, Yung, KKL, Yao, T |
| بيانات النشر: | //www.elsevier.com/locate/yexnr United States |
| سنة النشر: | 2012 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Animals, gamma-Aminobutyric Acid - physiology, Synaptic Transmission - physiology, Symporters - metabolism, Receptors, GABA-A - metabolism, Rats, Sprague-Dawley, Purkinje Cells - metabolism - physiology, Organ Culture Techniques, Inhibitory Postsynaptic Potentials - physiology, Cerebellum - metabolism - physiology, Brain-Derived Neurotrophic Factor - physiology, Newborn |
| الوصف: | The ability to regulate inhibitory synapses is a critical feature of the nervous system and a growing body of evidence indicates that brain-derived neurotrophic factor (BDNF) acutely modulates the efficacy of GABA synaptic transmission. Although the neuronal potassium-chloride cotransporter 2 (KCC2) has been implied in this BDNF-induced ionic plasticity, the reports about actions of BDNF on GABA signaling remain conflicting. Here we show dual effects of BDNF on GABAergic synaptic transmission in Purkinje neurons in rat cerebellar slices. BDNF decreased the amplitude of evoked outward IPSCs postsynaptically. It induced a depolarizing shift in the reversal potential (E IPSC), which reduced the driving force for outward IPSCs. However, in the absence of KCC2 activity, BDNF directly potentiated rather than inhibited GABA A receptor, which was reflected by an increase in the amplitude of outward IPSCs. This action of BDNF coincided with its effect in increasing the amplitude of inward IPSCs. Furthermore, an interaction between GABA A receptor and KCC2 was revealed by co-immunoprecipitation. The effects of BDNF on both GABA A receptor and KCC2 were dependent on TrkB and also activation of cyclin-dependent kinase 5 (Cdk5). However, only the effect of BDNF on KCC2 activity was dependent on a rise of intracellular calcium. Taken together, these data highlight distinct actions of BDNF on KCC2 and GABA A receptor in the regulation of GABAergic synaptic transmission. © 2011 Elsevier Inc. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1090-2430 0014-4886 22178325 |
| Relation: | Experimental Neurology; http://www.scopus.com/mlt/select.url?eid=2-s2.0-84856550555&selection=ref&src=s&origin=recordpage; Experimental Neurology, 2012, v. 233 n. 2, p. 791-798; 798; WOS:000300123600027; eid_2-s2.0-84856550555; 791; http://hdl.handle.net/10722/145822; 233 |
| DOI: | 10.1016/j.expneurol.2011.11.043 |
| الاتاحة: | https://doi.org/10.1016/j.expneurol.2011.11.043 http://hdl.handle.net/10722/145822 |
| رقم الانضمام: | edsbas.55CB1A2A |
| قاعدة البيانات: | BASE |
| تدمد: | 10902430 00144886 22178325 |
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| DOI: | 10.1016/j.expneurol.2011.11.043 |