Academic Journal
Methotrexate treatment hampers induction of vaccine-specific CD4 T cell responses in patients with IMID
| العنوان: | Methotrexate treatment hampers induction of vaccine-specific CD4 T cell responses in patients with IMID |
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| المؤلفون: | Kummer, L.Y.L., Fernández Blanco, L., Kreher, C., Bos, A., Kuijper, L.H., Verstegen, N.J.M., van de Sandt, C.E., Konijn, V.A.L., Duurland, M.C., Menage, C., Jorritsma, T., Steenhuis, M., Hagen, R.R., van den Dijssel, J., de Jongh, R., Ashhurst, T., van Gils, M.J., Garcia-Vallejo, J.J., Claireaux, M., Stalman, E.W., van Dam, K.P.J., Wieske, L., Boekel, L., Wolbink, G., Tas, S.W., Rispens, T., Kuijpers, T.W., Eftimov, F., van Ham, S.M., Ten Brinke, A. |
| المصدر: | Kummer , L Y L , Fernández Blanco , L , Kreher , C , Bos , A , Kuijper , L H , Verstegen , N J M , van de Sandt , C E , Konijn , V A L , Duurland , M C , Menage , C , Jorritsma , T , Steenhuis , M , Hagen , R R , van den Dijssel , J , de Jongh , R , Ashhurst , T , van Gils , M J , Garcia-Vallejo , J J , Claireaux , M , Stalman , E W .... |
| سنة النشر: | 2024 |
| المجموعة: | Universiteit van Amsterdam: Digital Academic Repository (UvA DARE) |
| الوصف: | Objectives Methotrexate (MTX) is one of the most commonly used medications to treat rheumatoid arthritis (RA). However, the effect of MTX treatment on cellular immune responses remains incompletely understood. This raises concerns about the vulnerability of these patients to emerging infections and following vaccination. Methods In the current study, we investigated the impact of MTX treatment in patients with immune-mediated inflammatory disease on B and CD4 T cell SARS-CoV-2 vaccination responses. Eighteen patients with RA and two patients with psoriatic arthritis on MTX monotherapy were included, as well as 10 patients with RA without immunosuppressive treatment, and 29 healthy controls. CD4 T and B cell responses were analysed 7 days and 3-6 months after two SARS-CoV-2 messenger RNA vaccinations. High-dimensional flow cytometry analysis was used to analyse fresh whole blood, an activation-induced marker assay to measure antigen-specific CD4 T cells, and spike probes to study antigen-specific B cells. Results Seven days following two SARS-CoV-2 vaccinations, total B and T cell counts were similar between MTX-treated patients and controls. In addition, spike-specific B cell frequencies were unaffected. Remarkably, the frequency of antigen-specific CD4 T cells was reduced in patients using MTX and correlated strongly with anti-RBD IgG antibodies. These results suggest that decreased CD4 T cell activity may result in slower vaccination antibody responses in MTX-treated patients. Conclusion Taken together, MTX treatment reduces vaccine-induced CD4 T cell activation, which correlates with lower antibody responses.NL8900. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1136/rmdopen-2024-004664 |
| الاتاحة: | https://dare.uva.nl/personal/pure/en/publications/methotrexate-treatment-hampers-induction-of-vaccinespecific-cd4-t-cell-responses-in-patients-with-imid(b49849d4-c67e-4142-a917-367b8eea5cca).html https://doi.org/10.1136/rmdopen-2024-004664 https://hdl.handle.net/11245.1/b49849d4-c67e-4142-a917-367b8eea5cca https://pure.uva.nl/ws/files/213962609/Methotrexate_treatment_hampers_induction_of_vaccine-specific_CD4_T_cell_responses.pdf http://www.scopus.com/inward/record.url?scp=85205792525&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.55B75A66 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/rmdopen-2024-004664 |
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