Academic Journal
Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon.
| العنوان: | Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon. |
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| المؤلفون: | Volle, J.N., Chambon, G., Sayah, A., Reymond, C., Fasel, N., Gijs, M.A. |
| المصدر: | Biosensors and Bioelectronics, vol. 19, no. 5, pp. 457-464 |
| سنة النشر: | 2003 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Adsorption, Biosensing Techniques/instrumentation, Biosensing Techniques/methods, Green Fluorescent Proteins, Luminescent Proteins/analysis, Luminescent Proteins/chemistry, Membranes, Artificial, Oxidation-Reduction, Proteins/analysis, Proteins/chemistry, Reproducibility of Results, Sensitivity and Specificity, Silanes/chemistry, Silicon/chemistry, Spectrometry, Fluorescence/methods, Surface Properties |
| الوصف: | We present a silicon chip-based approach for the enhanced sensitivity detection of surface-immobilized fluorescent molecules. Green fluorescent protein (GFP) is bound to the silicon substrate by a disuccinimidyl terephtalate-aminosilane immobilization procedure. The immobilized organic layers are characterized by surface analysis techniques, like ellipsometry, atomic force microscopy (AFM) and X-ray induced photoelectron spectroscopy. We obtain a 20-fold enhancement of the fluorescent signal, using constructive interference effects in a fused silica dielectric layer, deposited before immobilization onto the silicon. Our method opens perspectives to increase by an order of magnitude the fluorescent response of surface immobilized DNA- or protein-based layers for a variety of biosensor applications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/14623470; info:eu-repo/semantics/altIdentifier/pissn/0956-5663; https://serval.unil.ch/notice/serval:BIB_736547BDF053 |
| DOI: | 10.1016/S0956-5663(03)00201-X |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_736547BDF053 https://doi.org/10.1016/S0956-5663(03)00201-X |
| رقم الانضمام: | edsbas.55A68722 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/S0956-5663(03)00201-X |
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