Academic Journal
Carbon substrate re‐orders relative growth of a bacterium using Mo‐, V‐, or Fe‐nitrogenase for nitrogen fixation
| العنوان: | Carbon substrate re‐orders relative growth of a bacterium using Mo‐, V‐, or Fe‐nitrogenase for nitrogen fixation |
|---|---|
| المؤلفون: | Luxem, Katja E., Kraepiel, Anne M. L., Zhang, Lichun, Waldbauer, Jacob R., Zhang, Xinning |
| المساهمون: | National Science Foundation, Simons Foundation, Princeton Environmental Institute, Princeton University |
| المصدر: | Environmental Microbiology ; volume 22, issue 4, page 1397-1408 ; ISSN 1462-2912 1462-2920 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2020 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary Biological nitrogen fixation is catalyzed by the molybdenum (Mo), vanadium (V) and iron (Fe)‐only nitrogenase metalloenzymes. Studies with purified enzymes have found that the ‘alternative’ V‐ and Fe‐nitrogenases generally reduce N 2 more slowly and produce more byproduct H 2 than the Mo‐nitrogenase, leading to an assumption that their usage results in slower growth. Here we show that, in the metabolically versatile photoheterotroph Rhodopseudomonas palustris , the type of carbon substrate influences the relative rates of diazotrophic growth based on different nitrogenase isoforms. The V‐nitrogenase supports growth as fast as the Mo‐nitrogenase on acetate but not on the more oxidized substrate succinate. Our data suggest that this is due to insufficient electron flux to the V‐nitrogenase isoform on succinate compared with acetate. Despite slightly faster growth based on the V‐nitrogenase on acetate, the wild‐type strain uses exclusively the Mo‐nitrogenase on both carbon substrates. Notably, the differences in H 2 :N 2 stoichiometry by alternative nitrogenases (~1.5 for V‐nitrogenase, ~4–7 for Fe‐nitrogenase) and Mo‐nitrogenase (~1) measured here are lower than prior in vitro estimates. These results indicate that the metabolic costs of V‐based nitrogen fixation could be less significant for growth than previously assumed, helping explain why alternative nitrogenase genes persist in diverse diazotroph lineages and are broadly distributed in the environment. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/1462-2920.14955 |
| الاتاحة: | http://dx.doi.org/10.1111/1462-2920.14955 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14955 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14955 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14955 https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14955 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.55979D2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/1462-2920.14955 |
|---|