Academic Journal
Mass cytometry analysis reveals altered immune profiles in patients with coronary artery disease
| العنوان: | Mass cytometry analysis reveals altered immune profiles in patients with coronary artery disease |
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| المؤلفون: | Kott, K.A., Chan, A.S., Vernon, S.T., Hansen, T., Kim, T., de Dreu, M., Gunasegaran, B., Murphy, A.J., Patrick, E., Psaltis, P.J., Grieve, S.M., Yang, J.Y., Fazekas de St Groth, B., McGuire, H.M., Figtree, G.A. |
| المصدر: | http://dx.doi.org/10.1002/cti2.1462. |
| بيانات النشر: | WILEY |
| سنة النشر: | 2023 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | atherosclerosis, immune signature, inflammation, mass cytometry, T regulatory cells |
| الوصف: | Objective. The importance of inflammation in atherosclerosis is well accepted, but the role of the adaptive immune system is not yet fully understood. To further explore this, we assessed the circulating immune cell profile of patients with coronary artery disease (CAD) to identify discriminatory features by mass cytometry. Methods. Mass cytometry was performed on patient samples from the BioHEART-CT study, gated to detect 82 distinct cell subsets. CT coronary angiograms were analysed to categorise patients as having CAD (CAD+) or having normal coronary arteries (CAD ). Results. The discovery cohort included 117 patients (mean age 61 12 years, 49% female); 79 patients (68%) were CAD+. Mass cytometry identified changes in 15 T-cell subsets, with higher numbers of proliferating, highly differentiated and cytotoxic cells and decreases in na€ıve T cells. Five T-regulatory subsets were related to an age and gender-independent increase in the odds of CAD incidence when expressing CCR2 (OR 1.12), CCR4 (OR 1.08), CD38 and CD45RO (OR 1.13), HLA-DR (OR 1.06) and Ki67 (OR 1.22). Markers of proliferation and differentiation were also increased within B cells, while plasmacytoid dendritic cells were decreased. This combination of changes was assessed using SVM models in discovery and validation cohorts (area under the curve = 0.74 for both), confirming the robust nature of the immune signature detected. Conclusion. We identified differences within immune subpopulations of CAD+ patients which are indicative of a systemic immune response to coronary atherosclerosis. This immune signature needs further study via incorporation into risk scoring tools for the precision diagnosis of CAD. ; Katharine A Kott, Adam S Chan, Stephen T Vernon, Thomas Hansen, Taiyun Kim, Macha de Dreu, Bavani Gunasegaran, Andrew J Murphy, Ellis Patrick, Peter J Psaltis, Stuart M Grieve, Jean Y Yang, Barbara Fazekas de St Groth, Helen M McGuire, and Gemma A Figtree |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2050-0068 |
| Relation: | http://purl.org/au-research/grants/nhmrc/GNT11359290; Clinical & Translational Immunology, 2023; 12(11):e1462-1-e1462-18; https://hdl.handle.net/2440/140936; Psaltis, P.J. [0000-0003-0222-5468] |
| DOI: | 10.1002/cti2.1462 |
| الاتاحة: | https://hdl.handle.net/2440/140936 https://doi.org/10.1002/cti2.1462 |
| Rights: | © 2023 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| رقم الانضمام: | edsbas.55567DD9 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20500068 |
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| DOI: | 10.1002/cti2.1462 |