Academic Journal
Static vs. dynamic electrostatic repulsion reversed phase liquid chromatography: solutions for pharmaceutical and biopharmaceutical basic compounds
| العنوان: | Static vs. dynamic electrostatic repulsion reversed phase liquid chromatography: solutions for pharmaceutical and biopharmaceutical basic compounds |
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| المؤلفون: | Mazzoccanti, Giulia, Gasparrini, Francesco, Calcaterra, Andrea, Villani, Claudio, Ciogli, Alessia |
| المساهمون: | Mazzoccanti, Giulia, Gasparrini, Francesco, Calcaterra, Andrea, Villani, Claudio, Ciogli, Alessia |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | basic compound, static ERRP, dynamic ERRP, hydrophobic cation additive |
| الوصف: | Many efforts have been made to separate basic compounds, which are challenging to resolve in reversed phase liquid chromatography. In this process, they are strongly retained and the peak shape undergoes significant distortion. The principal origin of this has been identified with the non-negligible interaction with residual deprotonated silanols. Consequently, all solutions that efficiently shield silanols are being sought. This review is an upgrade on the use of the electrostatic repulsion reversed phase (ERRP) approach: retention of bases, in protonated form, can be achieved by modulating the charge repulsion caused by the presence of positive charges in the chromatographic system. This study successfully (i) introduced fixed positive charges in the structure of stationary phases, (ii) used cationic and hydrophobic additives in the mobile phase, and (iii) used the ERRP-like approach employed at the preparative level for peptide purification. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000654483800001; volume:8; issue:5; numberofpages:12; journal:SEPARATIONS; http://hdl.handle.net/11573/1545851; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85105982786 |
| DOI: | 10.3390/separations8050059 |
| الاتاحة: | http://hdl.handle.net/11573/1545851 https://doi.org/10.3390/separations8050059 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.547C910A |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/separations8050059 |
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